骨桥蛋白与矿化液诱导牙髓干细胞向成骨细胞分化的对比研究

Comparison of osteopontin and mineralization culture medium in the induction of dental pulp stem cells into osteoblasts

目的:比较骨桥蛋白(OPN)和矿化液对牙髓干细胞(DPSCs)向成骨细胞分化的作用。方法:矿化液培养DPSCs作为矿化液组,添加适宜浓度OPN作为OPN组,茜素红染色法检测矿化结节的形成;RT-RCR检测骨涎蛋白(BSP)、Runt相关转录因子2(Runx-2)、骨钙蛋白(OCN)、Ⅰ型胶原(Col-1)等骨源性基因表达情况。结果:茜素红染色显示诱导培养28 d后2组出现的矿化结节数量相近(P>0.05)。OPN诱导培养7 d后2组DPSCs骨源性基因表达均成阳性,其中OPN组BSP基因表达水平高于矿化液组(分别为0.864±0.112和0.514±0.068,P<0.05);OPN组Runx-2基因表达水平低于矿化液组(分别为0.186±0.017和0.324±0.058,P<0.05);Col-1及OCN基因表达水平相近(P>0.05)。结论:OPN和矿化液对DPSCs的诱导能力相近。

Objective：To compare the osteogenic differentiation of dental pulp stem cells（DPSCs）induced by osteopontin（OPN）and mineralizing culture medium（MCM）.Methods：DPSCs were cultured with OPN（OPN group）and MCM（MCM group）respectively. The morphology of the DPSCs were observed under inverted microscope.The mineralize nodules were observed by alizarin red staining. RT-RCR was used to detect the mRNA expression of bone sialoprotein （BSP）,Runt-related transcription factor 2（Runx-2）,osteocal-cin（OCN）and collagen-1（Col-1）.Results：Similar number of mineralized nodules was found in the 2 groups（P 〉0.05）after 28 day culture.The mRNA expression level of BSP gene in OPN group was higher than that in MCMgroup（0.864 ±0.112 and 0.514 ±0.068, P 〈0.05）,while the expression level of Runx-2 gene in OPN group is lower than that in MCMgroup（0.186 ±0.017 and 0.324 ±0. 058,P 〈0.05）.The expression level of Col-1 and OCN genes in both groups were similar（P 〉0.05）.Conclusion：The capabilities of OPN and MCMin inducing osteogenic differentiation of DPSCs are similar.