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p75NTR对舌鳞癌Tca8113细胞凋亡的影响 被引量：3

The effects of p75NTR gene on the apoptosis of tongue squamous cell carcinoma Tca8113 cells
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摘要 目的:探讨p75NTR基因对舌鳞癌Tca8113细胞凋亡的影响。方法:流式细胞技术分选Tca8113细胞系中p75NTR阳性细胞及阴性细胞,对阳性细胞用脂质体瞬时转染荧光标记的p75NTR siRAN,实时定量RT-PCR检测p75NTR基因,western blot检测p75NTR蛋白表达;流式细胞仪Annexin V/PI双染色标记法进行凋亡检侧;MTT检测细胞增殖的影响。结果:p75NTR表达阳性的Tca8113细胞经p75NTR siRNA转染后p75TNR的表达被抑制;细胞凋亡率明显增加(P<0.05),细胞增殖率明显降低。结论:p75NTR在Tca8113细胞凋亡中具有调控作用。 Objective：To study the effect of p75NTR gene on the apoptosis of tongue squamous cell carcinoma Tca8113 cells. Methods：p75NTR ＋Tca8113 cells were isolated from Tca 8113 cell line and transfected by p75NTR siRNA using lipofectamine. p75NTR mRNA and protein expression was examined by real time RT-PCR and western blot respectively.Cell proliferation was stud-ied by MTT assay,cell apoptosis was examined by flow cytometry.Results：Proliferation of p75NTR ＋ cells was faster than that of p75NTR - cells.Transfection of p75NTR siRNA inhibited p75NTR mRNA and protein expression in p75NTR ＋ Tca8113 cells,inhibi-ted the proliferation and increased the apoptosis of the cells.Conclusion：p75NTR gene plays a role in the apoptosis of Tca8113 cells.
作者 张兆弢 佟冬冬 李青 张风河
机构地区 山东大学口腔医院口腔颌面外科 山东省口腔生物医学重点实验室
出处 《实用口腔医学杂志》 CAS CSCD 北大核心 2015年第1期15-19,共5页 Journal of Practical Stomatology
基金 山东省自然科学基金(编号:ZR2012HM055)
关键词 舌鳞癌细胞 P75NTR SIRNA p75NTR siRNA Tongue OSCC cells p75NTR siRNA
分类号 R739.8 [医药卫生—肿瘤]
  • 相关文献

参考文献11

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二级参考文献13

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共引文献2

同被引文献52

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实用口腔医学杂志
2015年 第1期

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