牙科浸泡着色氧化锆陶瓷的细胞毒性研究

An in vitro cytotoxicity study of colorized zirconia by immersion method

目的：评价5种过渡金属元素染色液浸泡着色氧化锆陶瓷的细胞毒性。方法：采用浸泡着色的方法制备NH4VO3、Fe Cl3、Co（NO3）3、Ni（NO3）3、Mo Cl35种过渡金属着色的氧化锆3Y-TZP瓷块,用瓷块浸提液体外培养小鼠成纤维细胞（L-929）3 d,观察细胞形态并用MTT法测试吸光度值评价各着色瓷块的细胞毒性。结果：各组细胞培养3 d后生长良好,形态正常,各实验组的毒性评级为0-I级。结论：5种染色液浸泡着色所得氧化锆陶瓷无细胞毒性。

Objective：To evaluate the cytotoxicity of colorized zirconia ceramics.Methods：Zirconia ceramic 3Y-TZP blocks were colorized by NH4 VO3 ,FeCl3 ,Co（NO3 ）3 ,Ni（NO3 ）3 and MoCl3 respectively.The cytotoxicity of colorized zirconia to L-929 cells was examined by immersion method and MTT assay.Results：During 3 days culture,L929 cells in all groups showed similar prolifera-tion and normal morphology.The toxicity gradation of all groups was 0 -I.Conclusion：Zirconia ceramics colorized with the 5 kinds of colorants has no cytotoxicity.