EZH2蛋白在卵巢上皮性肿瘤中的表达及其作用

Expression of EZH2 protein and its role in ovarian epithelial tumor

目的观察EZH2在不同卵巢组织中的表达,探讨其在肿瘤发展中可能发挥的作用。方法采用免疫组织化学染色检测15例正常卵巢组织、32例卵巢良性肿瘤、28例卵巢交界性肿瘤及50例卵巢癌中EZH2蛋白的表达,其中15例正常卵巢组织、23例卵巢良性肿瘤、23例交界性肿瘤及25例卵巢癌行RT-PCR检测。结果 1EZH2蛋白在卵巢癌细胞核中的表达明显高于正常卵巢组织(P<0.01)、卵巢良性肿瘤(P<0.01)及交界性肿瘤(P<0.05),且与肿瘤的分级和分期具有相关性(P<0.01,P<0.05);2 EZH2蛋白在卵巢肿瘤组织的细胞质中亦有表达,且良性肿瘤和交界性肿瘤中的表达高于卵巢癌(P均<0.05);3 EZH2蛋白在卵巢癌细胞核中的表达与Ki-67呈正相关性(r=0.40,P<0.01)。结论卵巢肿瘤组织细胞核及细胞质中均有EZH2蛋白的表达,且卵巢癌细胞核中EZH2蛋白的表达与肿瘤的分期及分级具有相关性,并参与细胞增殖。

Objective To explore the expression of EZH2 protein in different epithelial ovarian tumors and its possible role in tumor development.Methods Immunohistochemistry was used to detect EZH2 protein expression in 15 normal ovarian tissues,32 ovarian benign tumors,28 ovarian borderline tumors and 50 primary epithelial ovarian cancers. Real-time quantitative PCR （RT-PCR）was used to detect EZH2 mRNA expression in 15 normal ovarian tissues,23 ovarian benign tumors,23 borderline tumors and 25 epithelial ovarian cancers.Results ① EZH2 expressed highly in nucleus in epithelial ovarian cancers compared with normal ovarian tissue （P 〈0.01）,benign tumor （P 〈0.01）and borderline tumor （P 〈0.05）.EZH2 expression was significantly correlated with grade and staging of tumor in epithelial ovarian cancers （P 〈0.01,P 〈0.05）.② EZH2 protein expressed in cytoplasm in ovarian tumor,and it expressed highly in benign ovarian tumor and borderline tumor compared with epithelial ovarian cancers （P 〈0.05 ）.③ The nuclear expression of EZH2 protein in epithelial ovarian cancers was positively correlated with Ki-67 （r =0.40,P 〈0.01）.Conclusion EZH2 protein expresses in both nucleus and cytoplasm in ovarian tumors,and its elevated nuclear expression in epithelial ovarian cancers is associated with not only staging and grade of tumor,but also cell proliferation.