水稻nyc3基因序列多样性及表达量分析

Sequence Diversity of nyc3 Gene and Its Expression Profile in Rice

nyc3基因在叶绿素降解过程中起关键作用。本研究通过分析nyc3基因在533份水稻核心种质材料中存在的自然变异,发现了nyc3基因序列具有非常丰富的单核苷酸多态性,并在基因的转录区域共找到了28个SNPs,其中在启动子区域检测到10个SNPs,在5'UTR检测到2个SNPs,在3'UTR检测到16个SNPs。利用生物信息学软件,将核心种质中nyc3分为14个单倍型,筛选其中6个主要单倍型进行染色体画图,明确了各单倍型之间存在的SNP差异,同时确定28个SNPs在基因转录区域的分布。通过实验室构建的全基因组范围内水稻全生育期表达谱数据库,找到在不同时期、不同组织中nyc3的表达量,通过分析可以发现,nyc3的表达量伴随着水稻的生长在不断的升高,在水稻成熟期,叶片中nyc3的表达量达到最高值,充分说明nyc3在调控叶绿素降解上起到作用。

Abstract nyc3 gene plays a crucial role in the process of chlorophyll degradation. Through the analysis of natural variation of nyc3 gene in 533 accessions of core collection, there are very rich SNP diversities of nyc3 gene with twenty eight SNPs found in the transcriptional regions, which include 10 SNPs found in promoter region, two SNPs in the 5＇UTR and sixteen SNPs in the 3＇UTR. nyc3 genes in core collection are divided into fourteen haplotypes by bioinformatics software. Six major haplotypes are choosen to map their locations of SNPs on the chromosome, which made clear the differences of SNPs among the six haplotypes, as well as distribution of twenty eight SNPs in the transcriptional regions. Expression of rtyc3 gene in different growth stages and tissues are found through the CREP （Collection of Rice Expression Profiling） that was developed by the National Key Laboratory of Crop Genetic Improvement, the results indicated that the expression profile of nyc3 gene should be constantly increasing following up the growth of rice plant. The expression of nyc3 reached the highest value in leaves while the rice is getting mature, which might explain fully that nyc3 gene plays a role on regulation of chlorophyll degradation.