转ScALDH21基因棉花植株能提高棉花抗盐能力

Transgenic Sc ALDH21 Cotton can Enhance Cotton Salt Tolerance at the Atage of Seed Germination and Seedling Growth

来自极端耐旱齿肋赤藓（Syntrichia caninervis）的ALDH21（GQ245973）基因能否提高棉花耐盐性尚未见报道。本研究利用将Sc ALDH21基因导入新农棉1号的3个T4代转Sc ALDH21基因棉花株系为研究材料,通过不同Na Cl浓度处理转基因棉花种子及其幼苗,鉴定转基因种子萌发和幼苗生长时期的耐盐能力。研究结果表明：受体棉种子萌发耐受的Na Cl浓度为0-50 mmol/L,转基因棉花种子萌发耐受的Na Cl浓度为50-100 mmol/L,部分株系,如L38,能够耐受150 mmol/L Na Cl浓度的盐胁迫;随着盐胁迫浓度升高,种子萌发延迟,萌发率也有不同程度的降低,但转基因种子萌发率均优于受体种子。在高Na Cl浓度（100-150 mmol/L）胁迫下,转基因幼苗鲜重显著高于受体,其鲜重比受体高75%以上;转基因株系SOD和POD活性较受体植株增强。本研究结果表明,在盐胁迫条件下,转Sc ALDH21基因棉花表现出优良的生长和生理优势,转Sc ALDH21基因能提高棉花的抗盐能力。

Whether ScALDH21 （GQ245973）, isolated from extreme drought tolerant plant （Syrztrichia caninervis）, could improve salt tolerance has not yet been reported in cotton. In this research, three transgenic ScA LDH21 lines in T4 generations and their wilr type Gossypium hirsutum L. cv. Xinnongrnian No. 1 were employed to analyze their salt tolerance based on chatacteristics of seed germination and seedling growth parameters under the stress of different salt concentration. The results showed that seed germination of transgenic lines had higher tolerance to NaC1 concentration in ranges of 50-100 mmol/L, some transgenic line reached up to 150 mmol/L, such as L38 being the best line that could tolerate the stress of 150 mmol/L, while wild type cotton can tolerate salt stress in the ranges of 0-50 mmol/L. With the increase of salt concentration, the delay of seed germination exhibited, and germination rate was significantly decreased as well, whereas, the seed germination rate of transgenic lines was higher than that of the control （WT）. In the condition of high NaC1 concentrations （100-150 mmol/L）, fresh weight of seedling was significantly different. Fresh weight of transgenic seedlings was 75% more than that of WT. And SOD and POD activities of transgenic lines were increased much more than that of WT. The results showed that the transgenic plants harboring with ScA LDH21 had better growth and physiological advantages under the stress ofNaCI, which indicated that transgenic SeA LDH21 cotton should enhance the salt tolerance.