穴位埋线对老年性骨质疏松小鼠骨密度及骨生物力学的影响

Effects of catgut implantation at acupuncture points on bone mineral density and bone biomechanics in senile osteoporotic mice

目的本研究旨在分析穴位埋线对老年性骨质疏松小鼠(SAMP6)骨密度和骨生物力学性能的影响,进而探讨该类方法对于防治老年性骨质疏松症的作用效果。方法选取3月龄快速老化模型SAMP6小鼠共40只随机分为对照组1月组(C1组)10只、对照组2月组(C2组)10只、埋线1月组(埋1组)10只和埋线2月组(埋2组)10只。选同龄SAMR1小鼠20只作为正常老化同源对照组,即R1 1月组(R1组)、R1 2月组(R2组)各10只。选取双侧"肾俞"和"足三里"每两周进行一次穴位埋线,分别于1月后、2月后将小鼠摘眼球取血处死,取双侧股骨,进行骨密度及骨生物力学检测。结果 1埋2组小鼠股骨密度均显著高于C2组;R2组显著低于R1组,显著高于C2组。2小鼠股骨弯曲(断裂)强度:与C1组相比,R1组和埋1组小鼠股骨弯曲(断裂)强度显著增高;与C2组相比,R2组和埋2组显著增高;埋2组显著高于埋1组。小鼠股骨最大载荷:埋1组和埋2组小鼠股骨最大载荷显著高于C1组和C2组。小鼠股骨弹性模量:埋1组和埋2组小鼠股骨弹性模量均显著高于C1组和C2组;与R1组和R2组无差异。小鼠股骨横断面积:与R1组相比,R2组小鼠股骨横断面积显著性低下;埋1组显著高于埋2组。结论 "肾俞"和"足三里"穴位埋线对老年性骨质疏松症骨密度和生物力学性能有积极作用。

Objective To analysis the effect of catgut implantation at acupuncture points on bone mineral density and bone biomechanics in senile osteoporosis mice（ SAM P6）,and to explore the mechanism of preventing and treating senile osteoporosis.Me thods Forty senescence accelerated mice（ SAM P6） were randomly divided into 4 groups,including 10 in control group 1（ C1）,10 in control group 2（ C2）,10 in catgut implantation for 1 month group 1（ M1）,and 10 in catgut implantation for 2months group 2（ M2）.Twenty normal senescence mouse（ SAM P1） with the same age were selected as homologous control groups： 10 in 1 month group（ R1）,and 10 in 2 months（ R2）.The implantation mice were undertaken bilateral ＂Shenshu＂and＂Zusanli＂catgut implantation once in 2 weeks.The mice were executed after eyeball blood collecting in 1 month and 2 months,respectively.The bilateral femurs were collected for the bone mineral density and bone biomechanical detection.Re sults 1）BM D of the femurs was significantly higher in group M2 than that in group C2,lower in group R2 than group R1,but significantly higher than in group C2.2） The bone bending intensity showed that the femur bending strength in group R1 and M1 was significantly higher than that in group C1,group R2 and M2 were higher than group C2,and group M2 was higher than group M1.The maximal loading in group M1 and M2 was higher than that in group C1 and C2.The elastic modulus in group M1 and M2 was higher than in group C1 and C2,but it was not different comparing to that in group R1 and R2.The femur transaction area in group R2 was significantly lower than that in group R1,and group M1 were higher than group M2.Conclusion ＂Shenshu ＂ and＂Zusanli＂catgut implantation has beneficial effect on bone mineral density and bone biomechanical indicators of the femur in osteoporotic mice.