3~6岁儿童血浆β-胶原特殊序列和N端中段骨钙素变化规律探讨

The role of plasma beta-crosslaps and N-mid fragment of osteocalcin in healthy 3-6-yearold children

目的探讨3~6岁儿童血浆β-胶原特殊序列（β-Crosslaps）和N端中段骨钙素（N-MID）的变化规律。方法按分层随机抽样选取广州市越秀区妇幼保健院2013年2月至4月3~6岁健康体检儿童90例,排除10例不合格者,得男性2.5~3.5岁组、4.5~5.5岁组和女性2.5~3.5岁组、4.5~5.5岁组,每组各20例。查询记录各研究对象性别、年龄、身高、体重,计算其年龄别体重Z分（WAZ）、年龄别身高Z分（HAZ）。采集清晨空腹静脉血,集中检测骨代谢标志物β-Crosslaps和N-MID的血浆水平,检测方法为电化学发光免疫分析法（ECLIA）。结果用SPSS11.0分析各组β-Crosslaps、N-MID的变化规律,骨代谢生化指标之间的相关性及其与受试者年龄、身高、体重、WAZ、HAZ之间的相关性。结果 1男、女β-Crosslaps、N-MID水平两年龄组比较之间无显著差别（P〉0.05）,同年龄组不同性别之间无显著差别（P〉0.05）,3~6岁儿童血浆β-Crosslaps和NMID平均水平分别为（1.28±0.31）ng/ml,（64.44±15.47）ng/ml。2在男、女2.5~3.5岁年龄组中,β-Crosslaps与N-MID的相关性显著（P〈0.05）,r分别为0.520、0.648,在男女4.5~5.5岁组β-Crosslaps与N-MID相关性均不显著（P〉0.05）。3男性4.5~5.5岁年龄组血浆N-MID与WAZ显著相关（r=0.541,P〈0.05）,其余各年龄组男、女血浆β-Crosslaps、N-MID与年龄、身高、体重、WAZ、HAZ相关性不显著（P〉0.05）。结论 3~6岁儿童骨代谢生化指标水平处于稳定水平,随年龄变化的改变不显著,3~6岁儿童血浆β-Crosslaps和N-MID平均水平分别为（1.28±0.31）ng/ml,（64.44±15.47）ng/ml,且与HAZ、WAZ无显著相关性,年龄、身高、体重、WAZ、HAZ不能代表当前骨代谢状态。

Objective To investigate the role of plasma beta-crosslaps（ β-Crosslaps） and N-mid fragment of osteocalcin（ NM ID） in healthy 3-6-year-old children.Me thods Ninety healthy 3-6-year-old children were chosen with stratified sampling method from Guangzhou City M aternal and Child Health Hospital of Yuexiu District from February to April 2013.Ten of them were disqualified.Both males and females were divided into 2.5- 3.5 year group and 4.5- 5.5 year group,with 20 children in each group.The height and weight of these children were recorded,and WAZ and HAZ were counted.The fast venous blood samples were collected.Plasma concentrations of β-Crosslaps and N-M ID were measured using electrochemiluminescence immunoassay（ ECLIA）.All date were analyzed using a SPSS 11.0 software.The correlation among bone metabolic and biomechanical parameters and age,height,weight,WAZ,and HAZ of the children was analyzed.Re sults 1） The serum β-Crosslaps and NM ID showed no significant difference between the gender and age groups（ P〈0.05）.M ean plasma concentrations of β-Crosslaps and N-M ID in healthy 3-6-year-old children were 1.28 ± 0.31 ng / ml and 64.44 ± 15.47 ng / ml,respectively.2） The association between β-Crosslaps level and N-M ID was positive in 3- 4- year-old boys and girls（ P〈0.05）,and the r was 0.520 and 0.648,respectively.The association was not significant in 4- 6- year-old boys and girls（ P〈0.05）.3） N-M ID and WAZ was closely associated in 4- 6- year-old male group（ r = 0.541,P〈0.05）.Both β-Crosslaps and N-M ID were not significantly associated with age,height,weight,HAZ,and WAZ（ P〈0.05） in the rest groups.Conclusion Levels of bone biochemical markers are relatively stable in healthy 3-6-year-old children.M ean plasma concentrations of β-Crosslaps and N-M ID in healthy 3-6-year-old children are 1.28 ± 0.31 ng / ml and 64.44 ± 15.47 ng / ml,respectively.Height,weight,age,HAZ,and WAZ can not reflect the present status of bone metabolism.