苏铁叶黄酮类化合物的检测与提取工艺研究

Extraction and Determination of Flavonoids from Leaves of Cycas revoluta

目的：以苏铁叶为研究材料，开展黄酮类化合物的检测，同时对其提取工艺进行优化。方法：以UV法检测的苏铁叶总黄酮得率为评价指标，在单因素试验的基础上，采用L9（3^4）正交试验，优化提取工艺条件。结果：定性检测显示，苏铁叶中含黄酮、二氢黄酮等黄酮类化合物组分。研究结果表明，最佳工艺参数：提取温度为80℃，乙醇浓度为70％，料液比为1．25，提取时间为1．5h。在此条件下总黄酮的平均得率为1．67％。结论：该工艺合理，重复性好，能有效提高苏铁叶总黄酮得率。

Objective： To study the extraction and determination of flavonoids from leaves of Cycas revoluta. Methods ： The total flavonoids was determined by UV. The yield of total flavonoids was used as index, the extraction condition was optimized by single factor test and orthogonal design L9 （3^4）. Results： The results showed that the leaves of C. revoluta contained varied flavonoids, including flavones, dihydrogen flavones etc. The optimal extraction conditions were as the following： temperature of 80 ℃, ethanol concentration of 70%, solid-liquid ratio of 1：25 and time 1.5 h. With this method, the extraction ratio of total flavonoids was 1.67%. Conclusion： The established method is reasonable, reproducible, and can effectively improve the yield of total flavonoids from C. revoluta leaves.