卷丹百合鳞茎高效离体再生体系的研究

Study on High- Efficient in vitro Regeneration from Bulb Scales of Lilium lancifolium Thunb

目的:以湖南主栽卷丹百合的鳞茎为起始外植体,建立卷丹百合鳞茎高效离体再生体系。方法:比较消毒方法、不同6-BA浓度、培养基组合和环境条件等对鳞茎污染率、不定芽的诱导、伸长和生根的影响。结果:用8%NaClO消毒15 min配合0.1%HgCl_2消毒15~20 min后,消毒效果较好,鳞片成活率高;温度为26±2℃,光照强度为2000 Lx条件下,6-BA浓度为2.5~3.5 mg·L^(-1),鳞片萌芽最快,不定芽生长最健壮;0.5 mg·L^(-1)6-BA+0.2 mg·L^(-1)NAA+1.0 mg·L^(-1)2,4-D培养基组合有利于不定芽的生长;0.5 mg·L^(-1)NAA培养基组合最有利于不定芽的生根,移栽成活率高达100%。结论:建立此体系为卷丹的脱毒苗培育和品种改良提供一定技术支持。

Objective： To establish a high-efficient in vitro regeneration system of bulb scales from Lilium lancifolium using the bulb scales from L. lancifolium as the initial explants. Methods ： The effect of sterilization, different concentrations of 6-BA, medium formulations, light intensity and temperature on pollution of bulb scales and induction, elongation and rooting of adventitious buds were compared. Results： The results showed that the optimal sterilization was using 8% NaClO for 15 min and 0. 1% HgCl2 for 15 -20 min. The optimal adventitious buds induction formulation was MS base medium with 2. 5 ~ 3.5 mg·L^-16-BA, and the temperature was 26 ~ 2℃ with 2000 Lx light intensity. The combination of 0. 5 mg·L^-16-BA ＋ 0. 2 mg·L^-1 NAA ＋ 1.0 mg·L^-12, 4-D benefited the elongation of adventitious buds. And 0. 5 mg·L^-1 NAA was the best combination of hormones for rooting, and survival rate of transplanting was 100%. Conclusion： The regeneration system provides a technical support for virus free seedling and variety improvement of L. lancifolium.