微波与超声波提取贻贝中嘌呤物质的方法研究

Comparative study of microwave and ultrasonic extraction methods of purine substances in mussels

以贻贝为原料,用微波和超声波对贻贝中嘌呤提取工艺进行了研究,探讨了两种方法的最佳提取条件和参数。结果表明,微波辅助萃取最佳工艺参数为:液料质量比40∶1,萃取时间为20 min,萃取温度65℃,嘌呤总提取率达1.695 8 mg/g;超声波提取条件为液料质量比80∶1,提取150 min,嘌呤总提取率为1.681 6 mg/g。与超声波提取方法相比,微波辅助萃取法具有萃取时间短、提取率高、节省溶剂等优点。

The microwave and ultrasonic methods were used to extract mussel respectively,and their extraction conditions were optimized. The results showed that the optimal conditions of the microwave extraction are as follows： mass ratio of extraction solvent to raw material 40∶1,microwave irradiation time 20 min and temperature 65 ℃. For the ultrasonic extraction,they are as follows： mass ratio of extraction solvent to raw mateial 80∶1,microwave irradiation time 150 min. The purine extraction rates are 1. 695 8 mg/g and1. 681 6 mg/g for the microwave and ultrasonic extraction methods under the optimal extraction condtions,respectively. Compared with ultrasonic method,microwave assisted extraction offers many advantages,such as a short extracting time,high efficiency,smaller volumes of chemical solvents.