巴中产栀子对大鼠肝损伤模型保肝降酶药理效应研究

PHARMACOLOGICAL EFFECT OF LIVER PROTECTION AND ENZYME REDUCTION OF GARDENIAE FRUCTUS FROM BAZHONG IN RAT LIVER DAMAGE MODEL

目的研究巴中产栀子对大鼠肝损伤模型保肝降酶药理效应。方法采用大鼠皮下注射四氯化碳(CCl4)制备肝损伤模型、分别灌胃给予栀子水溶性有效部位、70%醇溶性有效部位,速率法测定天冬氨酸氨基转移酶(AST)和丙氨酸氨基转移酶(ALT),观察巴中、湖北、江西和江津产栀子对CCl4导致的大鼠肝损伤模型降低AST和ALT作用。结果四个产地栀子无论是水溶性有效部位,还是70%醇溶性有效部位均具有非常显著的降低AST和ALT作用(与肝损伤模型组Model比较,分别为P<0.001、P<0.01、P<0.02、P<0.01);栀子水溶性有效部位比70%醇溶性有效部位降低AST、ALT的效果更好,但是无显著性差异(P>0.05)。巴中产栀子提取物降低AST、ALT效果比江西、湖北、江津产栀子更好(尤其水溶性有效部位),巴中产栀子水溶性有效部位对肝损伤大鼠AST活性降低作用显著优于江津产栀子水溶性有效部位(P<0.05),非常显著优于湖北产栀子水溶性有效部位(P<0.01),极显著优于江西产栀子水溶性有效部位(P<0.001);巴中产栀子水溶性有效部位降低ALT作用与湖北、江西、江津产栀子比较有进一步降低的趋势,但是无显著的差异(P>0.05);巴中产栀子70%醇溶性有效部位降低AST、ALT作用比江西、湖北、江津产栀子更有优势,但是无显著性差异(P>0.05)。结论根据栀子对大鼠CCl4肝损伤模型保肝降酶效应研究结果证明巴中产栀子的品质更优、疗效更佳。巴中产栀子作为川产道地药材的开发研究值得各级政府和社会各界的广泛关注。

Objective： To explore the pharmacological effect of liver protection and enzyme reduction of gardeniae fructus from Bazhong in rat liver damage model. Methods： Preparing rat liver injury model by subcutaneous injection of CCl4, administrating with water extraction and 70% ethanol extraction of gardeniae fructus by intragastric, respectively, determing the activity of AST and ALT by rate method, the reduced activity of AST and ALT on rats with damage liver by CCl4 of gardenia fructus from Bazhong, Hubei, Jiangxi and Jiangjin were investigated. Results： Water or 70% alcohol extraction of gardenia from Bazhong, Hubei, Jiangxi andJiangjin could significantly reduce the activity of AST and ALT （comparing with model group, P 〈 0.001, P 〈 0.01, P 〈 0.02, P 〈 0.01, respectively）; Water extraction was better than 70% ethanol extraction, but no significant difference （P 〉 0.05）. The effect of extraction in gardenia from Bazhong that reduced activity of AST and ALT was significantly better than those from Jiangxi, Hubei, and Jiangiin （special for water extraction）. The effect of water extraction in gardenia from Bazhong that reduced activity of AST was significantly better than that from Jiangjin （P 〈 0.05）, highly significantly better than that from Hubei （P 〈 0.01）, extremely better than that from Jiangxi （P 〈 0.001）. The effect of water extraction in gardenia from Bazhong that reduced activity of ALT was better than that from Jiangjin, Hubei Jiangxi, but no significant difference （P 〉 0.05）. The effect of 70% ethanol extraction in gardenia from Bazhong that reduced activity of AST and ALT was better than that from Jiangjin, Hubei Jiangxi, but no significant difference （P 〉 0.05）. Conclusion： Quality of gardeniae fructus from Bazhong is better than from Jiangxi, Hubei, and Jiangjin, gardeniae fructus from Bazhong is well worth focusing by government and society.