纳米金增加阿霉素耐药肝癌细胞株敏感性的实验研究

Gold nanoparticles re-sensitizing adriamycin-resistant hepatocellular carcinoma cells:an experimental study

目的:观察纳米金(GNP)人耐药肝癌细胞株耐药性的逆转作用。方法:采用氯金酸柠檬酸三钠还原法制备并鉴定;分别用GNP与阿霉素(ADM)组单独或联合作用于ADM耐药的肝癌细胞株Hep G2/ADM,以未处理的Hep G2/ADM细胞为对照,用MTT法检、流式细胞术检测细胞的增殖与凋亡情况;紫外分光光度计检测Hep G2/ADM细胞经ADM单独作用以及GNP与ADM联合作用后细胞内ADM浓度。结果:与对照细胞比较,ADM单独作用及GNP与ADM联合作用后,Hep G2/ADM的增殖均明显抑制、凋亡率明显升高,但后者的作用明显强于前者(均P<0.05),而GNP单独作用对细胞的增殖与凋亡无明显影响(均P>0.05);GNP+ADM作用后,Hep G2/ADM细胞内的ADM含量较ADM单独作用后的ADM含量明显增加[(2.92±0.13)μg/L vs.(1.68±0.74)μg/L,P<0.05]。结论:GNP可增加Hep G2/ADM细胞对ADM的敏感性,该作用可能与其增加Hep G2/ADM细胞内的ADM浓度有关。

Objective： To investigate the drug resistance reversing effect of gold nanoparticles（GNPs） on drug-resistant human hepatocellular carcinoma cell line.Methods： GNPs were synthesized by reducing hydrogen tetrachloroaurate using tri-sodium citrate, and identified. Adriamycin（ADM）-resistant hepatocellular carcinoma Hep G2/ADM cells were treated with GNP and adriamycin（ADM）, alone or in combination, using Hep G2/ADM cells without any treatment as a control,and then the cell proliferation and apoptosis were determined by MT assay and flow cytometry, respectively. The intracellular ADM concentration of Hep G2/ADM cells at er exposure to ADM alone or GNP plus ADM were determined by ultraviolet-visible spectrophotometer.Results： Compared with control cells, the cell proliferation was inhibited and apoptosis rate was increased significantly in Hep G2/ADM cells treated with ADM along or combination with GNP, and these effects were more remarkable in the latter than in the former（all P0.05）. The intracellular ADM concentration in Hep G2/ADM cells exposed to GNP plus ADM was significantly higher than in those exposed to ADM alone [（2.92±0.13） μg/L vs.（1.68±0.74） μg/L, P0.05].Conclusion： GNP can enhance the sensitivity of Hep G2/ADM cells to ADM, and this ef ect may be related to its increasing the intracellular ADM accumulation in Hep G2/ADM cells.