摘要
目的研究重组质粒pc DNA3.1/Sur P/Trail联合吉西他滨诱导胰腺癌细胞凋亡的能力。方法将人胰腺癌细胞株SW1990分为4组,第1组转染重组质粒联合吉西他滨(联合组),第2组单纯转染重组质粒(重组质粒组),第3组单纯加入吉西他滨(吉西他滨组),第4组未加任何处理(空白对照组)。通过Western blotting检测各组细胞中Trail蛋白表达的情况,并用流式细胞仪检测SW1990胰腺癌细胞凋亡率。结果 (1)重组质粒组、联合组均有Trail蛋白表达,吉西他滨组、空白对照组则无Trail蛋白表达;(2)联合组SW1990细胞凋亡率显著高于其他各组(P<0.05);重组质粒组和吉西他滨组SW1990细胞凋亡率显著高于空白对照组(P<0.05);重组质粒组与吉西他滨组比较,差异无统计学意义(P>0.05)。结论重组质粒pc DNA3.1/Sur P/Trail与吉西他滨联可显著提高SW1990胰腺癌细胞的凋亡率。
Objective To study the synergistic effect of the recombinant plasmid pcDNA3 . 1/SurP/Trail and gemcit-abine to induce apoptosis in pancreatic cancer cells. Methods The human pancreatic cancer cell line SW1990 were di-vided into four groups, the recombinant plasmid combined gemcitabine group （combination group）, the recombinant plasmid group, gemcitabine group and the control group. The expression of Trail was detected by Western blotting meth-od and analyzed the apoptosis of SW1990 cells by double labeling flow cytometry. Results （1） There was Trail protein expression in recombinant plasmid group and combination group, while no in gemctitabine group and control group;（2） The apoptosis rate of SW1990 cells in combination group was significantly higher than the other groups （ P0 . 05 ） . Conclusion Combination of recombinant plasmid pcDNA3 . 1/SurP/Trail and gemcitabine could signifi-cantly increase the rate of apoptosis of SW1990 pancreatic cancer cells.
出处
《胃肠病学和肝病学杂志》
CAS
2015年第1期72-74,共3页
Chinese Journal of Gastroenterology and Hepatology
