同一个体来源正常与炎性牙周膜干细胞生物学性能的比较

Comparasion of the biological characteristics of normal and inflammatory periodontal ligament stem cells from the same donor

目的:比较同一个体来源正常与炎性牙周膜干细胞的生物学性能。方法:分别分离培养同一个体来源正常牙周膜干细胞(h PDLSCs)及炎性牙周膜干细胞(i-h PDLSCs)。流式细胞仪鉴定两种干细胞表面抗原标记;MTT法、克隆形成率检测并比较两者的增殖能力;茜素红染色及油红O染色检测并比较其分化能力;RT-PCR检测成骨相关基因COL-1、Runx-2、BSP-mRNA及成脂相关基因LPL与PPARγmRNA的表达水平。结果:i-h PDLSCs及h PDLSCs均具有间充质干细胞特性,i-h PDLSCs增殖能力强于h PDLSCs,但分化能力明显弱于h PDLSCs(P<0.05);两者成脂能力比较无统计学差异(P>0.05)。结论:炎症微环境对牙周膜干细胞的生物学性能有一定的影响。

AIM：To compare the biological characteristics of inflammatory periodontal ligament stem cells （i-hPDLSCs）and healthy periodontal ligament stem cells （hPDLSCs）from the same donor.METHODS：i-hP-DLSCs and hPDLSCs were isolated from the same donor.Their colony-forming ability,proliferative capacity,cell sur-face antigens and differentiation ability were evaluated and quantified for statistical analysis.RESULTS：Both stem cells have highly proliferative and differentiative potential.i-hPDLSCs had a significantly higher rate of successive cul-ture and proliferation than hPDLSCs（P〈0.05）.hPDLSCs had a higher rate of cell osteogenic differentiation than i-hPDLSCs （P〈0.05）.The adipogenic potential of i-hPDLSCs and hPDLSCs showed no statistical difference.CON-CLUSION：Inflammatory environment influences the biological properties of periodontal ligment stem cells.