建立以CFSE标记细胞微球为参照检测人外周血EPC数量的方法及其临床意义

Using CFSE-labeled cell beads as control for enumeration of EPC by flow cytometry

建立以CFSE标记细胞微球做参照,以流式单平台检测EPC数量的方法并评价其临床应用效果。使用流式单平台计数,利用CFSE染色的荧光细胞微球作为内参照,分析标本中的EPC的数量,并与体外克隆计数相比较。基于流式单平台利用CFSE标记细胞微球参照检测人外周血EPC数量和体外克隆计数的结果一致,但是相对体外克隆方法来说,我们建立的方法实验时间和成本明显降低,技术要求也相应降低。基于流式单平台利用CFSE标记细胞微球参照检测人外周血EPC数量的方法能够更快更准确的检测EPC数量,节约时间和成本,更适合临床常规使用。

To establish a single platform of flow cytometry technology for EPC enumeration, and evaluate the effectiveness in terms of accuracy, and cost and time consumption. Cell counting was performed on the current single platform, and CFSE stained-cell fluorescent microspheres were used as internal reference. The number of EPC in samples was quantitatively ana- lyzed, and then compared to the number of in vitro clones. The number of EPC in peripheral blood, based on CFSE labeled cells microspheres using single platform of flow cytometry detection was consistent with the number of in vitro clones. Howev- er, the time and cost consumption is greatly reduced compared to cloning method in vitro, and technical requirements are also reduced accordingly. The single platform of flow cytometry technology based on CFSE labeled cell, was a better and faster method for detection of the number of EPC in human peripheral blood. This method is more economical of time and cost compare to the previous methods, and more suitable for routine clinical use.