食管鳞癌组织PTK6表达临床意义研究

Expression of TPK6 and its clinical significance in esophageal squamous carcinoma tissues

目的探讨酪氨酸激酶-6(protein tyrosine kinase 6,PTK6)在食管鳞癌组织中的表达,及其与食管鳞癌发生发展及临床病理因素的相关性。方法采用免疫组织化学方法,检测2003-01-01-2005-12-01中山大学肿瘤防治中心胸外科切除的67例食管鳞癌及正常食管黏膜上皮组织中PTK6表达水平;在蛋白质和RNA水平检测7对手术切除的食管鳞癌及其癌旁组织标本中PTK6的RNA与蛋白表达情况。结果 PTK6在癌组织中的表达明显弱于癌旁正常黏膜组织,50.7%(34/67)的食管鳞癌组织中检测到PTK6蛋白高表达,73.1%(49/67)正常食管黏膜上皮组织中检测到PTK6蛋白高表达,两者差异有统计学意义,χ2=7.123,P=0.008。食管癌组织中PTK6表达水平与肿瘤浸润深度(χ2=0.647,P=0.03)和病理分级(χ2=0.577,P<0.001)密切相关,PTK6低表达组5年总生存率为31.9%,显著低于PTK6高表达组的77.3%,差异有统计学意义,P=0.021。RT-PCR及蛋白质印迹结果证实,无论是在基因水平还是蛋白水平,PTK6在食管鳞癌组织中均呈低表达。结论 PTK6在食管鳞癌的发生发展中起着重要作用,可能作为食管癌判断预后的独立预测因素。

OBJECTIVE To investigate the expression of PTK6 in esophageal squamous cell carcinoma （ESCC） ,and analyze the relationship between PTK6 and prognosis of ESCC patients and clinicopathologic characteristics. METHODS Using immunohistochemistry, we performed a retrospective study of the PTK6 expression levels on 67 archival ESCC par-affin-embedded samples;Using RT-PCR and Western blotting,we evaluated the PTK6 protein and RNA expression levels in 7 pairs of surgically resectable esophageal tissues and their adjacent tissue samples. RESULTS In 67 cases of ESCC and ＇adjacent noncancerous epithelial esophageal tissues, the PK6 expression levels were lower in ESCC tissues significantly than in normal esophageal tissues. We observed high expression of PTK6 in 34 of 67（50.7%） ESCC and in 49 of 67 （73.1%） of adjacent esophageal tissues, respectively, the difference was statistically significant （X^2= 7. 123, P= 0. 008）. In esophageal tissues,PTK6 expression levels were closely related to the depth of tumor invasion （X2 = 0. 647, P = 0.03） and pathologic stage（X2=0. 577, P〈0. 001）. In the primary Escc category, high PTK6 expression patients had better 5-year overall survival than low PTK6 expression patients （31. 9 % vs 77. 3 %, P= 0. 021）. Both RT-PCR and Western blot confirmed that the PTK6 expression levels were lower in ESCC than that in normal esophageal tissues at the genetic level and protein level. CONCLUSION PTK6 plays an important role in the process of tumorigenesis and development of ESCC,which would be as an independent predictor for prognosis of ESCC.