PPARγ在绒毛组织中的表达与早期自然流产的关系

PPARγ expressed in villi in the organization and the relationship with spontaneous abortion

目的研究过氧化物酶体增殖物激活受体γ(PPARγ)蛋白在正常早孕及早期自然流产患者绒毛组织中的差异表达,探讨PPARγ与早期自然流产的关系。方法应用免疫组化PV两步法、Western blot法检测PPARγ蛋白在正常早孕妇女(对照组,n=40)和早期自然流产患者(实验组,n=40)绒毛组织中的表达。结果免疫组化显示PPARγ蛋白主要在两组绒毛组织中细胞滋养层细胞及绒毛外滋养层细胞中表达;Western blot法显示PPARγ蛋白在实验组绒毛组织中的表达较对照组高,差异有统计学意义(P<0.01)。结论 PPARγ对妊娠早期滋养细胞浸润起负性调节作用,PPARγ蛋白表达水平增高,可能与自然流产的发生有关。

Objective To investigate the protein expression of PPARγ in villi of first trimester spontaneous abor-tion. Methods To detect the expression of PPARγ in villi of normal first trimester pregnant women （ control group,n=40） and first trimester spontaneous abortion （experimental group,n=40） by immunohistochemistry and Western blot. Results PPARγ localisation was researched by immunohistochemiscal methods;and in control and experimental group,the protein was observed in both villous and extravillous trophoblast nuclei. Western blot analy-sis showed that in villi of experimental group,expression of PPARγ was significantly increased than that of control group （P〈0. 01）. Conclusion PPARγ may play an important role in the modulation of thophoblast invasion,a significantly enhanced expression of PPARγ is identified in villous and extravillous trophoblast of miscarriage pa-tients may be one factor linked to spontaneous abortion.