奇异变形杆菌螺纹迁徙生长现象研究

Study on Whorl Swarming Growth Phenomenon of Proteus Mirabilis

为探讨奇异变形杆菌(PM)螺纹迁徙生长现象产生的原因。通过对细菌波动培养的接种时间、湿度、接种手法、培养时平板放置、外加磁场和酸碱度等因素的改变,观察PM螺纹迁徙生长现象。结果显示在密封罐里进行波动培养的平板中出现螺纹迁徙生长现象的频率较高,不论什么时候接种、接种手法如何、磁场的有无、培养时平板放置的方式如何,细菌在迁徙生长过程都能表现出螺旋迁徙生长现象,而且细菌的螺旋环旋转的方向都是逆时针。实验结果证明,PM螺纹迁徙生长需要特定的酸碱度环境,它的螺纹迁徙生长现象产生可能与其遗传特性和地球的自转有关。

The present paper is aimed to explore the origins of Proteus mirabilis（PM）whorl swarming growth phenomenon.The whorl swarming growth phenomenon of PM was observed by changed bacterial culture inoculation time,humidity,vaccination practices,cultured flat placement,magnetic field,pH and other factors.Bacterial ring spiral direction of rotation is counterclockwise and the volatile growth process of PM was whorl swarming growth phenomenon.Spiro fluctuation phenomenon was of high frequency in the sealing tanks by cultured anytime inoculation,wherever inoculation technique applied or not,the presence or absence of the magnetic field,and wherever the dish position was.The experimental results showed that the whorl swarming growth phenomenon of PM requires specific pH environment,in which the facts may be relative to its genetic characteristics and the Earth＇s rotation.