间充质干细胞移植对脑损伤大鼠神经生化标志物分泌的调节

Regulation of Transplantation of Human Umbilical Cord Blood Derived Mesenchymal Stem Cells on Secretion of Neural Biochemistry Marker after Traumatic Brain Injury in Rats

本研究探讨人脐血间充质干细胞(HUCBMSCs)移植对大鼠创伤性脑损伤(TBI)模型的神经特异性蛋白分泌的调节和脑保护作用机制,旨在为脑损伤的干细胞治疗提供理论依据。研究中将用Brdu标记的HUCBMSCs经鼠尾静脉植入大鼠TBI模型。检测和比较假伤组、损伤组和移植组大鼠血浆生化指标(NSE、S100β蛋白、LDH、CK)的改变。采用HE染色观察损伤组、假伤组及移植组大鼠脑损伤周边区组织光镜下形态学变化。在整个实验过程中4组大鼠均未使用免疫抑制剂。实验结果表明移植组大鼠均未发生移植相关的死亡。损伤组大鼠在损伤后早期即可见NSE、S100β蛋白、LDH、CK指标有所升高,各时间点与假伤组比较差异有统计学意义(P<0.01)。HUCBMSCs移植组大鼠在损伤后上述生化指标在早期有所增高,但随着时间进展,移植组血清NSE、S100β蛋白、LDH、CK浓度下降,与损伤组比较差异有统计学意义(P<0.05)。组织学检测可见移植组脑组织病理改变较损伤组轻微。通过研究我们认为未应用免疫抑制剂的情况下,经尾静脉注射HUCBMSCs能减少血清NSE、S100β蛋白、LDH和CK的分泌,有效促进组织损伤的修复,有助于神经功能的恢复。

This research was to study the regulation of intravenous administration of human umbilical cord blood mesenchymal stem cells（HUCBMSCs）on secretion of neural specific protein in rats after traumatic brain injury（TBI）,and to explore its mechanisms promoting the recovery of neurological function.The TBI models of rats were established.We then injected HUCBMSCs,labelled by Brdu（5-bromo-2-deoxyuridine）,into the TBI rats via the tail vein using modified Feeney free-falling method.The levels of neural biochemical indicators（serum S100βprotein,NSE,LDH,CK）of rats were detected in shamed group,injury group and HUCBMSCs-transplanted group.And the morphological changes of brain tissue of rats in the three groups were observed by using HE staining under light microscope.During the whole experiment no immunosuppressant was used for the four groups.From the research,transplant-related death of the rats was not found in transplantation group.In the injury group,rises were found in contents of serum S100βprotein,NSE,LDH,CK in the early stage after the rats were injured,which were much higher than those in shamed group at correspondent time point（P〈0.01）.In HUCBMSCs-transplanted group,although these biochemistry indexes were found rising for a short period in the early stage,along with the time,these indexes were obviously lower than in those injury group（P〈0.05）.Under light microscopy pathological changes of rats in HUCBMSCs-transplanted group were much slighter than those in injury group.It was well concluded that in the situation of no immuno-suppressants,the intravenous-injected HUCBMSCs could reduce the secretion of serum S100βprotein,NSE,LDH,CK,promote the repair of tissue injury effectively,and promote the functional recovery of neurons.