肉桂醛对UVA照射后体外成纤维细胞表达MMP-1,MMP-3和MAPK信号的影响

Effect of Cinnamic Aldehyde on Expression of MMP-1,MMP-3 and MAPK Signal in Human Dermal Fibroblasts after UVA Irradiation

目的探讨肉桂醛对UVA照射后体外皮肤成纤维细胞表达基质金属蛋白酶-1（MMP-1）、基质金属蛋白酶-3（MMP-3）和丝裂原活化蛋白激酶（mitogen—activated protein kinases，MAPK）的影响。方法皮肤成纤维细胞在体外培养后，在培养基中加入不同浓度（0，5，10，20和40μM）肉桂醛，24h后MTT法检测细胞活性。皮肤成纤维细胞经肉桂醛预处理24h后再予UVA照射，Westernblot法检测MMP-1，MMP-3，ERK，JNK，p38磷酸化蛋白水平。结果0—40μM肉桂醛对成纤维细胞的活性无明显影响（P均〉0．05）。肉桂醛能抑制UVA照射后成纤维细胞MAPK信号蛋白ERK，JNK，p38的活化，其也能抑制成纤维细胞表达MMP-1和MMP-3，且各浓度间作用差异均有统计学意义（P均〈0．05）。结论肉桂醛可能通过抑制UVA照射后成纤维细胞MAPK信号蛋白的活化来抑制MMP-1和MMP-3的表达，减少胶原降解，延缓皮肤光老化。

Objective To investigate the effect of cinnamic aldehyde on expression of matrix metalloproteinase-1 （ MMP- 1 ） , matrix metalloproteinase-3 （MMP-3） and mitogen-activated protein kinases （MAPK） signal in human der- mal fibroblast after UVA irradiation. Methods Human dermal fibroblasts were treated with cinnamic aldehyde of different concentration （0,5,10,20 and 40μM） for 24 hours. The cell viability was determined using the MTT method. After the UVA irradiation, The MMP-1 and MMP-3 protein expression level was detected by Western blot. The phospholylation level of MAPK signal, including phospho-ERK, phospho-JNK and phospho- p38, was also detected by Western blot. Results Cinnamic aldeyde （0 - 40μM） has no effect on cell viability of fibroblasts （all P 〉 0.05 ）. Cinnamic aldeyde decreased MMP-1 and MMP-3 expression in fibroblast in a dose-denpendent manner（ P 〈 0.05 ）. Furthermore, the phosphorylation of MAPK signal was inhibited by cin- namic aldeyde dose-dependently （ P 〈 0.05 ）. Conclusion Cinnamic aldeyde maybe decreased MMP-1 and MMP-3 expression through suppressing phosphorylation of MAPK signal in fibroblasts after UVA irradiation. Cinnamic aldeyde appear to have the potential to decrease collagen degradation and delay skin photo-aging.