克拉霉素、多西环素对雌性BALB/c小鼠下生殖道解脲脲原体及其生物膜的影响

Clarithromycin,Doxycycline,and their Combination Therapy for Ureaplasma Urealyticum and Its Biofilm in the Lower Genital Tract of Female BALB / c Mice

目的探讨克拉霉素、多西环素对雌性BALB/c小鼠下生殖道解脲脲原体标准株血清4型(UU4)及其生物膜的清除作用。方法构建UU4感染BALB/c小鼠下生殖道生物膜动物模型7d后,将小鼠分为空白组、感染组、克拉霉素组、多西环素组及两者联合用药组,除空白组、感染组小鼠外,分别用上述药物治疗7d和14d,并于治疗结束时及治疗结束后10d时取宫颈组织行扫描电镜(SEM)和荧光显微镜等检查。结果 SEM下克拉霉素、多西环素及两者联合治疗7d后小鼠宫颈仍可见游离的UU及其生物膜,治疗14d后无游离的UU,但生物膜结构仍然存在。结论 UU4形成的生物膜结构不能被常规剂量的克拉霉素和多西环素清除。

Objective To study the role of Clarithromycin and Doxycycline against Ureaplasma urealytieum serovar4 and its biofilm in the lower genital tract of female BALB/c mice. Methods Seven days after establishing the BALB/c murine model of female lower genital tract with Ureaplasma urealyticum serovar4, mice were divided into 5 groups, that are blank control group, U. urealyticum infection group, clarithromycin treatment group, dox- ycycline treatment group and combination treatment group. The mice were treated by Clarithromyein, Doxycyc- line and clarithromycin pius doxycycline for 7 or 14 days except the first two groups. In the second and tenth day after treatment, the cervical tissues were collected and then examined through scanning electron microscope （SEM） and fluorescence microscope. Results Observed through SEM after 7 days＇ therapy of Clarithromy- cin, Doxycycline and their combination therapy, bacteria and biofilms were still adhering to the cervical muco- sa. After 14 days ,there were still biofilms on the cervical tissues but bacteria could not be observed anymore. Conclusion The biofilms formed by Ureaplasma urealyticum serovar4 could not be eliminated by regular do- ses of Clarithromycin and Doxycycline.