蛇床子素和黄芩苷对黑素细胞树突生成的影响

Effects of the Herbal Active Ingredients Osthole and Baicalin on Dendricity of Melanocytes in Co-cultrue of Human Melanocytes and Keratinoeytes

目的探讨蛇床子素和黄芩苷对黑素细胞树突生成的影响。方法体外培养人黑素细胞和角质形成细胞共培养体系,利用MTT法检测蛇床子素和黄芩苷对共培养细胞存活率的影响;倒置显微镜下观测黑素细胞树突生成;逆转录聚合酶链反应(RT-PCR)检测黑素细胞树突调控基因Rac和Rho mRNA的表达。结果蛇床子素(20μg/m L)有促黑素细胞树突生长的作用(P<0.01);而黄芩苷(20μg/m L)对树突生长无明显影响(P>0.05)。蛇床子素有激活树突调控基因Rac的作用(P<0.01),而对Rho基因无影响(P>0.05)。黄芩苷对树突Rac和Rho基因均无影响(P>0.05)。结论蛇床子素通过激活Rac基因促进树突的生成。黄芩苷对对树突生成无影响。

Objective To study the effects of Osthole and Baicalin on dendricity of melanocytes in co-cultrue of human melanocytes and keratinoeytes in vitro. Methods The co-culture model of human melanocytes and keratino- cytes were prepared. MTr method was used to detect the cell viability in co-culture of human melanocytes and keratinocytes after treatment with Osthole and Baicalin. The morphology in human melanocytes was observed by inverted microscope. RT-PCR was applied to examine the expression of Rac mRNA and Rho mRNA on melano- cytes before and after Osthole and Baicalin were added. Results Compared to control group, Osthole （20p.g/ mL ） could up-regulate the expression of Rac mRNA after 12 h and 24 h culture significantly（ P 〈 0.01 ） , while the effects of Osthole on the expression of Rho mRNA were not significant （P 〉 0.05 ）. Baicalin（20p^g/mL） - treated cells exhibited almost the same levels of Rac mRNA and Rho mRNA expression compared with control group after 12 h and 24 h culture （ P 〉 0.05 ）. Conclusion The results suggested that Osthole activated the expression of Rac mRNA, resulting in melanocyte dendrite elongation. Baicalin has no influence on the dendrite elongation.