沙鼠肝泡球蚴组织中微血管密度、血管内皮生长因子的表达及意义

Expressions of MVD-CD34 and VEGF in Hepatic Alveolar Hydatid Tissue in Gerbil Model and Their Clinical Signii cances

目的研究沙鼠肝泡球蚴组织中微血管密度(MVD)-CD34及血管内皮生长因子(VEGF)的表达及意义。方法将60只健康的长爪沙鼠随机平均分为2组,即实验组和对照组。实验组沙鼠采用开腹肝穿刺法,每只鼠接种原头节悬混液0.1 m L,对照组以相同的方法接种等量的PBS。分别于接种后第20、40、60、80、100 d时每组各处死6只沙鼠,实验组分别取泡球蚴组织和泡球蚴周围肝组织,对照组取正常肝组织。免疫组织化学法观察各时间点沙鼠肝泡球蚴组织中MVD-CD34及VEGF的表达情况。结果感染泡球蚴沙鼠肝脏中均见大小不等的团块状囊泡组织,部分播散至腹腔。在感染的各时间点,泡球蚴组织中均可见到MVD-CD34和VEGF的表达,定位于肝泡球蚴组织"外囊"囊壁内皮细胞的细胞浆。在感染后第20 d、40 d、60 d、80 d和100 d时,泡球蚴组织中MVD分别为(9.83±3.87)/HP、(25.33±6.71)/HP、(34.50±5.50)/HP、(37.67±5.71)/HP和(44.67±4.93)/HP,与泡球蚴周围肝组织〔0/HP、(1.17±0.98)/HP、(3.50±1.38)/HP、(5.83±2.71)/HP、(8.83±2.48)/HP〕和正常肝组织(均为0)相比,差异均有统计学意义(P<0.05)。各时间点泡球蚴组织中VEGF免疫组织化学评分分别为(2.95±0.46)分、(3.90±0.68)分、(4.27±1.05)分、(5.33±0.95)分和(4.50±0.81)分,与泡球蚴周围肝组织〔(1.07±0.63)分、(1.38±0.75)分、(1.55±0.83)分、(1.67±0.47)分、(2.10±0.55)分〕和正常肝组织〔(1.02±0.83)分、(1.12±0.63)分、(1.26±0.26)分、(1.20±0.74)分、(1.21±0.28)分〕相比,差异均有统计学意义(P<0.05)。结论血管生成可能是泡球蚴浸润性生长的机制之一,VEGF可能促进沙鼠肝泡球蚴组织血管新生。

Objective To detect the expressions of microvessel density（MVD）-CD34 and vascular endothelial growth factor（VEGF） in hepatic alveolar hydatid tissue in gerbil model and explore their clinical significances. Methods Sixty health gerbils were randomly equally divided into two groups, an experimental group and a sham operation group, each gerbil was given liver vaccination by opening their abdominal. Each gerbil in the experimental group was injected with approximately 400 echinococcus protoscoleces（0.1 m L）, and each gerbil in the sham operation group received a corresponding volume of physiological saline. Six gerbils were sacrificed on day 20, 40, 60, 80, and 100. The hepatic alveolar hydatid tissue（AE） and its surrounding liver tissue（HSAE） were collected from the experimental group and the normal liver tissue（NL） was collected from the sham operation group, and the expressions of MVD-CD34 and VEGF were detected by immunohistochemistry staining（En Vision method）. Results Echioncoccus multilocularis hydatid tissues were observed over the liver and in the partly abdominal cavity in the experimental group each gerbil by general observation. The expressions of CD34 and VEGF were observed in the AE at each time point after infection and located in the cytoplasmic of endothelial cells. The number of MVD-CD34 of AE at each time point in the AE was（9.83±3.87） /HP,（25.33±6.71） /HP,（34.50±5.50） /HP,（37.67±5.71） /HP and（44.67±4.93） /HP, respectively, which were significantly higher than those in the HSAE 〔0/HP,（1.17±0.98） /HP,（3.50±1.38） /HP,（5.83±2.71） /HP, and（8.83±2.48） /HP, respectively〕and NL（all were 0）, P〈0.05. The point of VEGF at each time point in the AE was 2.95±0.46, 3.90±0.68, 4.27±1.05, 5.33±0.95, and 4.50±0.81 respectively, which were significantly higher than those in the HSAE（1.07±0.63, 1.38±0.75, 1.55±0.83, 1.67±0.47, 2.10±0.55, respectively） and NL（1.02±0.83, 1.12±0.63, 1.26±0.26, 1.20±0.74, 1.21±0.28）, P0.05. Conclusion Angiogenesis might be involved in infiltrated growth of alveococcus, and VEGF might contribute to angiogenesis of alveolar hydatid tissue.