缺氧/复氧大鼠心肌中IL-1β浓度的动态变化及意义

Dynamic changes of IL-1β in rat myocardium during hypoxia/reoxygenation transition

目的:探讨大鼠心肌中白细胞介素-1β(IL-1β)在心肌缺氧/复氧过程中的动态变化及其意义。方法:采用Langendorff方法制备离体大鼠心肌缺氧/复氧模型。40只雄性SD大鼠随机分为对照组(A组)和缺氧/复氧组(H/R组)。H/R组按照复氧时间分为H/R 0.5 h、H/R 1 h、H/R 2 h组(B、C、D组)(n=10)。连续纪录各组左室发展压(LVDP)、左心室发展压最大上升/下降速率(±dp/dtmax)的变化,ELISA检测各组心肌IL-1β和肌酸激酶同工酶(CK-MB)的浓度,RT-PCR检测心肌IL-1βmRNA的表达水平,光镜观察心肌结构。结果:与对照组相比,H/R组大鼠LVDP、±dp/dtmax值均降低(P<0.05),心肌中IL-1β的含量及IL-1βmRNA的表达明显升高(P<0.05),CK-MB浓度上升(P<0.05),随复氧时间的延长以上指标的变化更明显,B、C、D组间两两比较,差异均有统计学意义(P<0.05),H/R 2 h组心肌中的IL-1β、CK-MB的浓度以及IL-1βmRNA的表达量与A、B、C组相比,达到最高(P<0.05)。结论:大鼠心肌缺氧/复氧后IL-1β在心肌组织中的表达上升并引起心肌再灌注损伤。

Objective： To investigate the expression profile of interleuki-1β（ IL-1β） in rat myocardium at different time points during hypoxia / reoxygenation（ H / R） transition. Methods： The isolated Langendorff perfused rat heart model was established. Forty SD rats were randomly divided into sham group（ A group） and hypoxia / reoxygenation group（ H / R group）. The H / R group rats were subdivided into H / R 0. 5 h group（ B group）,H / R 1 h group（ C group）,H / R 2 h group（ D group） according to reoxygenation time. The1 eft ventricular development pressure（ LVDP）,maximal rates of increase / decrease of the left ventricular pressure（ ± dp / dtmax） were continuously recorded. The concentration of interleukin-1β（ IL-1β） and creatine kinase-MB（ CK-MB） in myocardium was measured by ELISA. The mRNA expression of IL-1β in myocardium was determined by RT-PCR. Microstructure of myocardium was observed under light microscopy. Results： The value of LVDP and ± dp / dtmaxin hypoxia / reoxygenation group rat were significantly lower than that in sham group（ P〈 0. 05）. The expression of IL-1β and CK-MB at protein level and the expression of IL-1β at mRNA level in hypoxia / reoxygenation group were higher than that in sham group（ P 〈0. 05）. There were significant differences of the above parameters among H / R 0. 5 h,1 h,2 h group（ P〈 0. 05）. The concentration of IL-1β and CK-MB,the mRNA expression of IL-1β were higher in H / R 2 h group than that of other groups（ P〈 0. 05）. Conclusion： The high expression of IL-1β in myocardium after myocardial hypoxia / reoxygenation in rats might lead to. ischemia / reperfusion injury.