Survivin和Ki67在胰腺内分泌肿瘤中表达及预后价值的评估

The study of the expression and the prognostic value of Survivin and Ki67 in pancreatic endocrine tumors

目的:探讨Survivin及Ki67与胰腺内分泌肿瘤(PETs)临床病理特征和预后的关系。方法:免疫组化检测25例正常胰腺组织和81例PETs组织芯片中Survivin、Ki67的表达情况,并分析其与预后的关系。结果:1PETs中Survivin、Ki67表达显著高于正常胰腺组织(P<0.01);2Survivin及Ki67在PETs中的表达均与病理分级和TNM临床分期有关(P<0.05),与肿瘤大小、发生部位及功能分类无相关性。另外,胞核Survivin阳性表达与淋巴结转移具有相关性(P<0.05);3Ki67高增殖指数与胞核Survivin阳性表达显著相关,与胞浆Survivin相关性无统计学意义。结论:Survivin和Ki67在PETs中均有表达,并且与PETs的临床病理特征关系密切,可作为评判PETs预后的新型指标。其中,胞核Survivin的阳性表达较胞浆阳性表达更具有诊断意义,其与淋巴结转移显著相关,是提示PETs预后不良的重要依据。

Objective： To explore the relationship between the expression of Survivin and Ki67 with prognosis of pancreatic endocrine tumors（ PETs）. Methods： Immunohistochemistry for Survivin and Ki67 was performed in 25 cases of normal pancreatic tissues and 81 cases of PETs by tissue microarrays and to observe the expression and evaluate the relationship with prognosis. Results： 1The expression of Survivin and Ki67 in PETs was significantly higher than that in normal pancreatic tissues（ P 〈0. 01）; 2The expression of Survivin and Ki67 in PETs was correlated with tissue grading and the TNM-staging（ P 〈0. 05）,but not related with tumor size、location and functional status. In addition,the expression of nuclear Survivin was association with lymph node metastasis（ P 〈0. 05）. 3The high expression of Ki67 was related with the expression of nuclear Survivin,but not related with the expression of cytoplasmic Survivin. Conclusion： Survivin and Ki67 were both expressed in PETs,which were closely related to the clinical pathological characteristics. They could be used as new indicators in the evaluation of prognosis of PETs. The expression of Survivin in nucleus had more diagnostic significance than that in cytoplasm,and that could be highly correlated with lymph node metastasis,which would be used as a new marker of poor prognosis.