金银花黄酮对小鼠免疫调节作用的研究

Effects of Lonicera Japonica flavone on immunomodulation in mice

目的:探讨金银花黄酮对小鼠血清免疫酶活性与淋巴器官的抗氧化作用。方法:将50只昆明小鼠随机分为5组(n=10):正常组、模型组、低剂量组、中剂量组、高剂量组。模型组和正常组小鼠生理盐水灌胃,低、中、高剂量组分别给予金银花黄酮100 mg/kg、200 mg/kg、400 mg/kg体重灌胃。连续灌胃7周后,除正常组注射生理盐水,其他各组小鼠均采用25 mg/kg体重的地塞米松连续3 d皮下注射,造成免疫抑制后继续灌胃1周,处死全部小鼠,取血和胸腺、脾脏,称重胸腺和脾脏,计算小鼠脏器指数,测定血浆中酸性磷酸酶(ACP)、碱性磷酸酶(AKP)、溶菌酶(LSZ)活力,以及胸腺和脾脏匀浆中单胺氧化酶(MAO)、总抗氧化能力(T-AOC)、超氧化物歧化酶(SOD)、丙二醛(MDA)。结果:金银花黄酮能显著提高免疫抑制小鼠的脏器指数,增加免疫抑制小鼠血清ACP、AKP和LSZ活力,提高免疫抑制小鼠的脾脏、胸腺组织T-AOC和SOD活性,而明显降低MAO和MDA含量。结论:金银花黄酮能显著调节小鼠血清免疫酶活性,提高淋巴器官的抗氧化功能,表明具其有良好的免疫调节功能。

Objective： To study immunomodulating activity of Lonicera Japonica flavone by investigating immune enzymatic activity of serum and ant-ioxidized activity of lymphoid organs in mice. Methods： Fifty KM mice were randomly divided into control group,model group,low dose group,middle dose group and high dose group（ n = 10）,respectively. And low dose group,middle dose group and high dose group were given Lonicera Japonica flavone with 100 mg / kg,200 mg / kg and 400 mg / kg every day,respectively,while control group and model group were administered with NS. After continuously giving drug 7 weeks,other groups were injected with Dexamethasome（ Dex： 25 mg / kg） for 3 days by subcutaneous injection,but the control group were treated with NS. And after giving Lonicera Japonica flavone 1 week simultaneously,organ indexes,the activity of acid phosphatase（ ACP）,alkaline phosphatase（ AKP） and lysozyme（ LSZ） in serum,and the content of monoamine oxidase（ MAO）,total antioxidant capacity（ T-AOC）,total superoxide dismutase（ SOD） and malondialdehyde（ MDA） in lymphoid organs in mice were tested,respectively. Results： Lonicera Japonica flavone could significantly improve the organ indexes,and significantly improve the activity of ACP、AKP and LSZ in serum,and significantly improve the contents of T-AOC and SOD,but reduce that of MAO and MDA in lymphoid organs in immunosuppressed mice. Conclusion： Lonicera Japonica flavone can significantly improve the activity of immune enzyme in serum and the anti-oxidized activity of lymphoid organs in mice. It suggests that Lonicera Japonica flavone has a good immunomodulatory effects.