布地奈德对大鼠变应性鼻炎最轻持续炎症反应模型IL-12表达的影响

Effect of budesonide on the expression of IL-12 in animal model of minimal persistent inflammation of allergic rhinitis in rats

目的:研究布地奈德对大鼠变应性鼻炎(AR)最轻持续炎症反应(MPI)模型的影响并观察IL-12的表达变化。方法:60只健康SD大鼠随机分为A、B、C、D共4组,每组15只。A组为阳性对照组即AR组,B组为实验组即MPI给药组,C组为阴性对照组即MPI不给药组,D组为空白对照组。首先以含卵清蛋白(OVA)和氢氧化铝凝胶的生理盐水混合液对A、B、C 3组行基础致敏和强化致敏,再分别用1%和0.01%的OVA溶液或生理盐水长期鼻腔激发,D组始终以生理盐水进行致敏和激发。第36天起每次激发前30min B组给予布地奈德喷鼻,其余各组给予生理盐水代替。观察豚鼠鼻腔激发后症状(喷嚏)变化并检测鼻黏膜中嗜酸粒细胞(EOS)浸润程度、上皮细胞内细胞间黏附分子1(ICAM-1)及IL-12的表达情况。结果:B组在以1%OVA溶液激发时,喷嚏平均次数明显多于D组(P<0.05),而与A、C组相比差异均无统计学意义(P>0.05);改用0.01%OVA溶液鼻腔激发并给药后,AR症状基本消失,与D组相比差异无统计学意义(P>0.05),鼻黏膜内仍有少量EOS浸润,并可见上皮细胞ICAM-1轻度表达,但与D组相比差异无统计学意义(P>0.05),B组IL-12阳性表达明显,与D组相比差异无统计学意义。结论:布地奈德可显著抑制AR模型迟发相炎症反应,提高大鼠AR MPI模型IL-12的表达。

Objective：To investigate the influence of budesonide on animal model of minimal persistent inflam mation （MPI） of allergic rhinitis in rats and to investigate the changes of interleukin 12 （IL-12） in nasal mucosa. Method：Sixty Sprague Dawley （SD） rats were randomly divided into four groups： group A （allergic rhinitis group） ,B （experimental group）,C（MPI model group） and D （bland group） respectively, with fifteen animals in each group. Rats from group A,B and C were sensitized intraperitoneally by injection of suspension of ovalbumin （OVA） and aluminum hydroxide in 0.9% physiological saline. Then, repeated local booster sensitization with dif- ferent concentration of OVA suspension （1 % and 0.01 % ） or physiological saline into the nasal cavity of those rats were performed. For group D, physiological saline was used only. From 36th day, group B were given budesonide treatment for three weeks. A,C and D group were given normal saline nasal spray. Symptoms （sneezing） of rats after antigen challenge were observed and the infiltration of eosinophils （EOS） together with the expression of in tereellular adhesion molecule 1 （ICAM-1） and IL-12 in the nasal epithelial ceils were also examined. Result^When challenged with 1% OVA,the sneezing number of rats in group B was increased markedly than that in group D （P〈0.05）. However, there was no difference between group B,A and C （P〉0.05）. When challenged with 0.01 % OVA and given budesonide ,the symptom of sneezing almost disappeared in group B just like that in group D and there was no difference between the two groups （P〉0.05）. Besides, there was still more EOS infiltrated in the nasal mucosa of rats in group C than that in group D（P〈0.05）. There was no expression of ICAM-1 in nasal epithelium of rats in group D,nevertheless,ICAM-1 was found mildly expressed in group C. IL-12 expression was significantly increased compared with group A and group C, and was no significantly difference compared with bland group（P〉0.05）. Conclusion：Budesonide significantly inhibited the late reaction of animal model of minimal persistent inflammation （MPI） of allergic rhinitis in rats and increase the expression of IL-12 in MPI model.