深圳市家禽感染常见禽流感病毒谱分析

The analysis of common avian influenza in infected poultry in Shenzhen city in 2013

目的监测家禽禽流感病毒感染状况,掌握家禽感染常见禽流感病毒的病毒谱,为制定科学的防控措施提供参考依据。方法采用RT-PCR技术分析对环境样本进行禽流感病毒普通型核酸检测,对普通型结果为阳性的样本再进行H5、H7、H9亚型禽流感病毒核酸检测。结果 2013年共采集环境样本310例,共检测出普通型禽流感病毒70例,禽类携带高致病性禽流感病毒阳性率为22.6%,其中H9亚型是深圳市禽类主要的感染禽流感病毒亚型。鸭是深圳市携带禽流感病毒率最高的禽类(58.8%),病毒携带率与其他禽类比较差异有统计学意义(χ2=12.212,P=0.002)。砧板、刀具相对其他环境样本病毒检测率最高,差异有统计学意义(χ2=4.431,P=0.045)。不同的喂养方式对禽类携带高致病性禽流感病毒影响不明显,差异无统计学意义(χ2=1.621,P=0.260)。结论 2013年深圳市禽类中存在H7、H9等禽流感病毒,H9亚型是禽类携带的主要亚型。

Objective To analyze the common avian influenza virus spectrum in infected poultry in Shenzhen city, and offer evidence for establishing scientific measures of prevention and control. Methods Reverse transcriptase polymerase chain reaction（RT-PCR） was used to detect avian influenza virus. And the positive samples would be further detected for the subtype of H5, H7, and H9 by RT-PCR. Results 310 environmental samples were collected in 2013 year. 70 samples were positive of avian influenza virus. Positive rate of high pathogenic avian influenza virus carrier of poultry was 22.6%; of which,H9 avian influenza virus was the main infected subtype. Ducks were the highest avian influenza virus carrier in poultry（58.8%）, which had statistical significant differences compared with virus carrier rates of other poultry（χ^2=12.212,P =0.002）. Chopping block and cutting tool had higher rates of virus carrier than other environmental samples, and the difference had statistical significance（χ^2=4.431, P =0.045）. Various feeding patterns made no obvious influence on status of high pathogenic avian influenza virus carrier of poultry, in which there was no statistical significance（χ^2=1.621,P =0.260）.Conclusion There were subtype of H7, H9 and other avian influenza virus in poultry of Shenzhen city in 2013; of which,H9 was the major subtype.