HCV细胞培养系统及其在中草药提取物抗HCV研究中的应用

HCV culture system and its application in antiviral research on Chinese herb extracts in vitro

应用中草药治疗丙型肝炎报道较多,然而这些研究大多采用中药复方制剂进行临床治疗,疗效局限。由于一种中药含有多种活性物质,某些物质之间在体内甚至有相互拮抗的作用,因此,对于治疗结果的作用机理很难阐述清楚。简述了HCV假病毒、复制子和全长细胞培养系统的研究现状,并对应用HCV RNA细胞模型研究中药单体或有效成分进行抗HCV实验研究的相关进展进行了综述,为中药治疗丙型肝炎研究提供新思路。

Many studies have shown that Chinese herb extracts could treat patients who suffer hepatitis C.However,the clinical efficacy is limited due to the use of traditional Chinese medicine （TCM）compounds.Because each drug contains multiple active substances,some of which may even act against each other in vivo,it is hard to clarify the mechanism of treatment.The current research on pseudotyped hepatitis C virus （HCV）,replicon,and cell culture system is summarized,and the advances in antiviral research on the monomers or active compo-nents of Chinese herbs in vitro based on HCV RNA models are reviewed,thus providing new ideas for TCM treatment of hepatitis C.