重症急性胰腺炎大鼠的早期细菌移位

Bacterial Translocation in Early Severe Acute Pancreatitis Rats

目的:建立大鼠重症急性胰腺炎(SAP)模型,观察FITC标记的大肠杆菌突破肠屏障及其发生细菌移位的时间及途径。方法:健康雄性Wistar大鼠50只,随机分为SAP组(n=30)和假手术组(n=20);造模前3 h用FITC标记的大肠杆菌给大鼠灌胃,分别在造模后2、3、4、6、8 h处死动物,分别取胰腺、肠系膜淋巴结制备组织匀浆;另取门静脉血及腹水,离心取上清,荧光化学发光检测仪检测标本中FITC的荧光强度,确定细菌移位出现的途径及时间。结果:与假手术组相比,SAP组于造模后3 h在腹水,4 h在胰腺组织,6 h在肠系膜淋巴结中检测出较高强度的荧光(P<0.05),并以腹水中的荧光强度为最高;门静脉血至造模后8 h仍未检测到细菌移位。结论:腹水和淋巴液可能是SAP早期细菌移位的重要途径,细菌可通过上述途径早期发生移位。

Objective To study the time and route of bacterial translocation when FITC-labeled E.coliwent through the intestinal barriers of rats with Severe Acute Pancreatitis（SAP）.MethodsFifty healthy malewistar rats with weight of（250 ± 30） g were randomly divided into a SAP group（n=30） and a sham-operatedgroup（n=20）. FITC-labeled E.coli were given by gavage 3 hours before surgery. The rats were killed at 2 h, 3 h,4 h, 6 h and 8 h after SAP was induced. Their pancreas and mesenteric lymph nodes were removed. The routeand time of bacterial translocation were examined.ResultsCompared to the sham-operated group, a higherfluorescence intensity（P 0.05） in ascitic fluid at 3 h, in pancreas at 4 h, and in mesenteric lymph nodes at6 h in SAP group was detected. The fluorescence intensity in ascitic fluid was the highest. But bacterial translo-cation in the portal vein blood 8 hours after SAP was did not detected.ConclusionSAP can lead to impair-ment of intestinal barrier. Ascites and lymphatic system may be the important channels for bacterial transloca-tion in early SAP.