新型基因重组藻胆蛋白对小鼠S180实体瘤COX-2表达的影响

Effect of New Recombinant Phycobiliprotein on Expression of COX-2 of Mice Treated with S180 Solid Tumor

目的探讨新型基因重组藻胆蛋白对小鼠S180实体瘤环氧化酶-2(COX-2)表达的影响。方法将小鼠腋下接种S180细胞荷瘤造模后,随机分为阴性组、环磷酰胺组、新型基因重组藻胆蛋白组[50mg/(kg·d)]、藻胆蛋白低、高浓度组[25mg(kg·d),100mg/(kg·d)],共5组,阴性组给予等量生理盐水,灌胃给药11天后处死,剥离肿瘤,采用免疫组化方法检测肿瘤组织中COX-2的表达。结果新型基因重组藻胆蛋白组、藻胆蛋白低、高浓度组突变型COX-2平均阳性表达率分别为28.64%、42.38%、31.68%,均低于阴性组66.12%(P<0.05)。结论新型基因重组藻胆蛋白可明显抑制COX-2表达,可能对COX-2表达有下调作用。

Objective To explore the effect of new recombinant phycobiliprotein on expression of cyclooxygenase - 2 （COX - 2） of mice treated with S180 solid tumor. Methods The tumor - bearing mice which were inoculated with S180 solid tumor were randomly di- vided into five groups： model group, cyclophosphamide group, new recombinant phycobiliprotein group [ 50mg/（ kg · d） ], low, high dose of phycocyanin group[ 25,100mg/（ kg · d） ], the same amount of saline was given to model group. After the intragastric administration for 11 days, the solid tumor was removed from each group,and the expression of COX - 2 was detected by immunohistochemical SABC meth- od. Results The average positive expression rates of COX - 2 among new recombinant phycobiliprotein group, low, high dose of phyco- cyanin group were respectively 28.64% ,42.38% ,31.68% , more lower compared with model group66.12% （P 〈 0.05 ）. Conclusion New recombinant phycobiliprotein may decrease expression of COX -2 significantly by down regulation effect.