摘要
目的研究在哮喘状态下,细胞外基质(extracellular matrix,ECM)对气道平滑肌细胞(airway smooth muscle cells,ASMCs)的免疫调节作用。方法健康雄性Wistar大鼠随机分为哮喘组(8只)及正常对照组(8只)。大鼠哮喘模型使用卵清蛋白(OVA)致敏及激发的方法建立。分离培养得到的大鼠ASMCs分别置于包被有LA及COL-1的细胞瓶中进行培养。结果Western blot法检测结果显示,eotaxin、TGF-β1的表达,哮喘空白组高于正常对照空白组(P<0.05),哮喘组内ECM的干预促进表达的增加(P<0.05),正常对照组表达差异无统计学意义(P>0.05)。结论 ECM影响哮喘ASMCs的免疫功能。
Objective To study extracellular matrix (ECM) in airway smooth muscle cells (ASMCs) immunomodulatory effects in asthma status. Methods Healthy male Wistar rats were randomly divided into asthmatic and control groups with eight in each group. Asthmatic rat model was established in using ovalbumin (OVA) sensitization and stimulation methods. ASMCs were isolated and cultured in the flasks, which coated with LA and COL- 1. Expression of eotaxin and TGF- β1 protein in ASMCs was detected by Western blot. Results Western blot showed that the expression level of eotaxin,TGF - β1 among the different groups was higher in Asthma group than in the normal control group (P 〈 0.05 ). Interventions ECM increased expression within asthma group ( P 〈 0.05 ). No difference was found in expression in control group (P 〉 0.05). Conclusion ECM can affect the immune function in asthmatic ASMCs.
出处
《医学研究杂志》
2015年第1期113-116,共4页
Journal of Medical Research