GSH-Px、IL-6及NF-κB在急性血糖波动的大鼠主动脉中的变化

Acute blood glucose fluctuation affects GSH-Px, IL-6, and NF-κB in aorta

目的探讨急性血糖波动对主动脉中谷胱甘肽过氧化物酶（GSH—Px）、白细胞介素-6（IL-6）水平及核因子-κB（NF—κB）表达的影响。方法间断或持续48h静脉输注50％葡萄糖注射液，建立急性血糖波动和高血糖动物模型。采用比色法检测主动脉匀浆中GSH—Px水平；酶联免疫吸附测定（ELISA）法检测IL-6的水平；免疫组化的方法检测主动脉内皮细胞核中NF—κB表达。结果急性血糖波动组主动脉中GSH—Px[（6．26±0．38）nmol／mgprot]表达较持续高糖组[（8．98±0．56）nmol/mgprot]及对照组[（10．02±1．10）nmol／mgprot]显著降低，急性血糖波动组IL-6[（20．56±3．78）pg／m1]水平较持续高糖组[（16．35±2．45）pg／m1]及对照组[（9．82±0．61）pg／m1]显著升高，NF—κB表达也显著升高。结论急性血糖波动能导致机体的氧化应激水平明显升高；启动NF—κB信号通路，炎症因子表达增加，从而损伤动脉。

Objective To investigate the effect of acute blood glucose on the level of glutathione peroxidase （GSH-Px） , interleukin-6 （ IL-6）, and the expression of nuclear factor-κB （NF-κB）. Methods The Wistar rats were infused intermittently or consistently with 50% glucose solution. The level of GSH- Px was investigated with colorimetry. The level of IL-6 was measured with enzyme-linked immunosorbent assay （ELISA）. The expression of NF-κB was investigated with immunohistochemisty. Results The level of GSH-Px in aorta homogenate in the acute blood glucose fluctuation group [（6. 26 ±0. 38） nmol/mgprot] was evidently lower than that of consistently high blood glucose group [ （ 8.98±0. 56 ） nmol,/mgprot ] and the control group [ （ 10. 02±1.10） nmolfmgprot ] （ P 〈 0. 05 ）. The level of IL-6 [ （ 20. 56±3.78 ） pg/ ml ] and the expression of NF-κB in the aorta in the acute blood glucose fluctuation group [ （ 16. 35±2.45 ） pg,/ml] were evidently higher than the other two groups （ P 〈0. 05）. Conclusions Acute glucose fluctuations induced the enhancing of oxidative stress and expression of inflammatory cytokines in the aorta, which leads to start NF-κB signaling pathway.