金叶女贞果实花青素的镇痛作用初探

Exploration on analgesia effect of anthocyanins from Ligustrum vicaryi fruit

目的:研究金叶女贞果实花青素的镇痛作用及其可能机制。方法:实验动物分为5组:生理盐水对照组,阿司匹林对照组,花青素高、中、低剂量组。通过小鼠热板法和醋酸扭体法,观察不同浓度花青素的镇痛作用;同时测定小鼠血清中超氧化物歧化酶(SOD)、NO和前列腺素E2(PGE2)水平,初步分析花青素可能的镇痛机制。结果:中、高浓度的花青素能提高小鼠痛阈,减少扭体次数;花青素处理组小鼠血清SOD活性增高,NO和PGE2含量降低。结论:金叶女贞花青素具有镇痛作用,其机制可能与提高小鼠抗氧化能力增强血清SOD活性,减少NO和炎性因子PGE2的生成有关。

AIM： To investigate the analgesic effect mechanism of anthocyanins from Ligus- trurn vicaryi. METHODS： Experimental animals were divided into 5 groups （n=8）： normal sa- line control group （NS）; aspirin control group （Asp） ; high-concentration anthocyanins group （ HA ） ; medium-concentration anthocyanins group （MA） ; low-concentration anthocyanins group （LA）. We observed the analgesia effect of different concentration anthocyanins by hot-plate test and acetic acid writhing test. The activity of superoxide dismutase （SOD） and the contents of nitric oxide （NO） and prostaglandin E_2 （PGE_2） in blood serum were determined to analysis the analgesia mechanism. RESULTS： The pain threshold of mice was enhanced and the times of twist body was decreased by medium-concentra- tion and high-concentration anthocyanins. The activity of SOD was increased and the contents of NO and PGE_2 were reduced in blood serum. CONCLUSION： Anthocyanins of Ligustrum vicaryi has definite analgesic effect, which was related to increasing the activity of SOD and de- creasing the contents of NO and PGE_2.