黄芪对急性肾缺血-再灌注大鼠细胞因子、游离钙水平及细胞周期蛋白D1基因表达的影响

Effect of Astragalus on Cytokines,the Level of Intracellular Calcium and the Expression of Cyclin D1 gene in Acute Renal Ischemia-Reperfusion Rats

目的:探讨黄芪对急性肾缺血-再灌注大鼠肾功能、细胞因子、游离钙水平和细胞周期蛋白(Cyclin D1)的影响。方法:90只SD雄性大鼠随机分为肾缺血-再灌注组(模型组)、假手术组(对照组)、黄芪组(实验组)模型,分别于再灌注后3,6,12h各留取10只取血及肾组织标本,比较各组肾功能、细胞因子、游离钙水平和Cyclin D1水平。结果:1与对照组相比,模型组6h后血肌酐(Cr)、尿素氮(BUN)均明显增加(P<0.01);实验组6h后BUN明显增加,12h后Cr增加,与对照组相比差异具有统计学意义(P<0.05);6h后模型组与实验组Cr差异具有统计学意义(P<0.05);12h后模型组与实验组BUN差异具有统计学意义(P<0.05)。2再灌注3,6,12h后模型组和实验组IL-6均高于对照组(P<0.01),但实验组低于模型组(P<0.01);模型组6h后IL-10低于对照组和实验组(P<0.05),其他组别间差异无统计学意义(P>0.05);再灌注3,6,12h后模型组和实验组TNF-α均高于对照组(P<0.01),但实验组低于模型组(P<0.01)。3模型组和实验组的[Ca2+]i自3h后即明显高于对照组(P<0.01),但实验组3,6h水平低于模型组(P<0.01),12h模型组和实验组差异无统计学意义(P>0.05);Cyclin D1水平,3h后模型组高于对照组(P<0.05),实验组3h与模型组差异有统计学意义(P<0.05),6h后与模型组差异无统计学意义(P>0.05)。结论:黄芪能够减轻缺血-再灌注大鼠钙超载,调控细胞因子释放,具有减轻大鼠肾脏损伤、保护肾功能的作用。

Objective：To investigate the effect of astragalus on cytokines in rat model with acute renal ischemia-reperfusion,as well as the changes of the level of intracellular calcium and the expression of Cyclin D1 gene.Methods：Ninety SD male rats were randomly divided into and renal ischemia-reperfusion group（model group）,sham operation group（control group）,and astragalus group（experimental group）.The blood and renal tissue samples were collected respectively at 3,6and 12 hafter reperfusion to determine renal function,cytokines,intracellular calcium level and Cyclin D1 level.Results：Compared with the control group,Cr and BUN of model group increased after 6h,the difference was statistically significant（P0.01）;BUN significantly in-creased in experimental group after 6h,and Cr increased after 12 hompared with the control group（P0.05）;The difference of Cr between model group and experimental group after 6h was statistically significant（P0.05）;so was BUN after 12h（P0.05）.IL-6of model group and experimental group at 3,6,12 hafter reperfusion were higher than those in the control group（P0.01）,but IL-6in experimental group was lower than that in the model group（P0.01）;IL-10 of model group after 6hwere lower than that in the control group and the experimental group（P0.05）,there was no significant difference between the other groups（P0.05）.TNF-αof experimental group and model group at 3,6,12 hafter reperfusion were higher than in the control group（P0.01）,but the TNF-αin experimental group was lower than that in the model group（P0.01）.[Ca2＋]I of model group and experimental group after 3hwas significantly higher than that of control group（P0.01）,but the levels of experimental group at 3hand 6h was lower than those in the model group（P0.01）,and there was no significant differences in 12 hbetween model group and experimental group（P0.05）;Cyclin D1 of model group after 3hwas significantly higher than that of control group（P0.05）,there was difference between experimental group and model group（P0.05）but the levels of experimental group after 6hwas no different significant from those in the model group（P0.05）.Conclusion：Astragalus could relieve the ischemic-reperfusion calcium overload of rat,release cytokines,and reduce renal damage.