催乳合剂对哺乳大鼠乳腺组织中AQP1和AQP3的影响

Effect of Lactation Mixture on Aquaporin 1 and Aquaporin 3 Protein Expression in the Lactating Rat Mammary Gland

目的观察催乳合剂对哺乳大鼠乳腺组织中AQP1和AQP3表达的影响,探讨催乳合剂增加泌乳量的机制。方法SD雌性大鼠30只,随机抽取10只,设为正常未哺乳大鼠组,剩余大鼠20只受孕后随机分为两组,正常哺乳组和催乳合剂催乳组,催乳合剂催乳组于产后第1天开始灌食催乳合剂,正常哺乳组于产后第1天开始灌食等量蒸馏水,均在哺乳第8天取乳腺组织,采用免疫组织化学方法检测正常未哺乳组和正常哺乳组大鼠乳腺组织中AQP1和AQP3的表达和定位,采用Western blot法检测比较3组AQP1和AQP3蛋白表达变化。结果在未哺乳组及正常哺乳组乳腺小叶组织及间质中AQP1蛋白均有表达,以表达于血管内皮细胞为主,AQP1蛋白在3组乳腺组织中表达无明显变化;在未哺乳组乳腺小叶组织及间质中未见AQP3蛋白表达,在哺乳期组乳腺小叶组织中AQP3蛋白表达明显,主要表达在乳腺腺泡上皮细胞胞质中。与未哺乳组相比,AQP3在正常哺乳组及催乳合剂催乳组乳腺组织中的表达均升高,尤其是在催乳合剂催乳组乳腺组织中升高更显著。结论催乳合剂增加泌乳量的机制与乳腺组织中AQP3密切相关。

Objective To investigate the mechanism of lactation mixture of increasing amount of lactation,we examined the ettect ot lactation mixture on aquaporin 1 and aquaporin 3 protein expression in the lactating rat mammary gland. Methods SD female rats were divided into three groups： virgin female rats group （n = 10） ,lactating rat treated with distill water groupfrom postpartum day 1 （n = 10） , and lactating rat treated with lactation mixture group from day 1 postpartum （rt = 10）. On days 8 postpartum lactating rat mammary gland were obtained. Immunohistochemistry was used to detect the AQP1 and AQP3 protein expression and localization in the mammary gland of virgin female rats and lactating rats treated with distill water. ]mmunoblotting was used to compare expression of AQP1 and AQP3 protein among three groups. Results In virgin female rats group and lactating rat treated with distill water group, AQP1 protein was expressed in the mammary lobular and stroma, mainly in vascular endothelial cells. AQP3 was not detected in virginfemales. AQP3 was found in mam- mary lobular in lactating rat, mainly in cytoplasm of mammary gland acinar epithelial cells. There was no significantly change of AQP1 pro- tein expression among three groups. Whereas compared with virgin female rats group, AQP3 expression was both increased in lactating rat treated with distill water groupandlactating rat treated with lactation mixture group, especially the latter increased more significantly. Conclusion The expression of AQP3 protein in the lactating rat mammary gland was closely related to the mechanism of lactation mixture increased amount of lactation.