摘要
目的:建立检测菊花及其炮制品种主要成分的方法,并对三批菊花及其炮制品中的主要成分绿原酸(C16H18O9)、木犀草苷(C21H20O11)、异绿原酸A(C25H24O12)进行含量测定。方法:色谱柱Agilent XBD C18(4.6 mm×250 mm,5.0μm),流动相乙腈/0.1%磷酸,流速1.0 m L/min,柱温25℃,检测波长348 nm。结果:三种化合物的分离度较好,理论塔板数较高,绿原酸:A=8.278 71C+4.935 05,R=0.999 9(n=7),浓度范围为8.656~86.56μg/m L;木犀草苷:A=15.866 45C-2.036 02,R=0.999 9(n=7),浓度范围为4.16~41.60μg/m L;异绿原酸A:A=8.514 90C+1.2142 8,R=1.0(n=7),浓度范围为18.92~189.20μg/m L,三种化合物的峰面积和浓度有良好的线性关系。结论:本方法能够准确快速地对菊花及其炮制中,主要成分进行定量分析,具有简便、稳定、准确等特点。
Objective: To establish a methods for determination of chlorogenic acid,luteolin,isochlorogenic acid A in Chrysanthemum and processed products by HPLC. Methods: The separation was performed on Agilent XBD C18( 4. 6 mm × 250 mm,5. 0μm),the mobile phase consisted of acetonitrile /0. 1% H3PO4 at flow rate of 1. 0 m L / min. The detection wavelength was set at 348 nm and column temperature was 25 ℃. Result: Those chemical constituents have been separated,chlorogenic acid: A =8. 278 71 C + 4. 935 05,R = 0. 999 9( n = 7),The linear rang was 8. 656- 86. 56 μg / m L; Luteoloside:A = 15. 866 45C- 2. 036 02,R = 0. 999 9( n = 7),The linear rang was 4. 16- 41. 60 μg / m L; isochlorogenic acid A: A = 8. 514 90 C + 1. 214 28,R = 1. 0( n = 7),The linear rang was 18. 92- 189. 20 μg / m L,the peak area and concentration of the three compounds have a good linear relationship. Conclusion: The method can accurately and quickly determination of chlorogenic acid,luteolin,isochlorogenic acid A in Chrysanthemum and processed products.
出处
《中国野生植物资源》
2015年第1期26-29,共4页
Chinese Wild Plant Resources
关键词
HPLC
菊花
绿原酸
木犀草苷
异绿原酸A
HPLC
Chrysanthemum
chlorogenic acid
luteoloside
isochlorogenic acid A