HPLC法测定菊花及其炮制品中主要成分的含量

Determination of Chemical Constituents in Chrysanthemum and Processed Products by HPLC

目的：建立检测菊花及其炮制品种主要成分的方法,并对三批菊花及其炮制品中的主要成分绿原酸（C16H18O9）、木犀草苷（C21H20O11）、异绿原酸A（C25H24O12）进行含量测定。方法：色谱柱Agilent XBD C18（4.6 mm×250 mm,5.0μm）,流动相乙腈/0.1%磷酸,流速1.0 m L/min,柱温25℃,检测波长348 nm。结果：三种化合物的分离度较好,理论塔板数较高,绿原酸：A=8.278 71C＋4.935 05,R=0.999 9（n=7）,浓度范围为8.656~86.56μg/m L;木犀草苷：A=15.866 45C-2.036 02,R=0.999 9（n=7）,浓度范围为4.16~41.60μg/m L;异绿原酸A：A=8.514 90C＋1.2142 8,R=1.0（n=7）,浓度范围为18.92~189.20μg/m L,三种化合物的峰面积和浓度有良好的线性关系。结论：本方法能够准确快速地对菊花及其炮制中,主要成分进行定量分析,具有简便、稳定、准确等特点。

Objective： To establish a methods for determination of chlorogenic acid,luteolin,isochlorogenic acid A in Chrysanthemum and processed products by HPLC. Methods： The separation was performed on Agilent XBD C18（ 4. 6 mm × 250 mm,5. 0μm）,the mobile phase consisted of acetonitrile /0. 1% H3PO4 at flow rate of 1. 0 m L / min. The detection wavelength was set at 348 nm and column temperature was 25 ℃. Result： Those chemical constituents have been separated,chlorogenic acid： A =8. 278 71 C ＋ 4. 935 05,R = 0. 999 9（ n = 7）,The linear rang was 8. 656- 86. 56 μg / m L; Luteoloside：A = 15. 866 45C- 2. 036 02,R = 0. 999 9（ n = 7）,The linear rang was 4. 16- 41. 60 μg / m L; isochlorogenic acid A： A = 8. 514 90 C ＋ 1. 214 28,R = 1. 0（ n = 7）,The linear rang was 18. 92- 189. 20 μg / m L,the peak area and concentration of the three compounds have a good linear relationship. Conclusion： The method can accurately and quickly determination of chlorogenic acid,luteolin,isochlorogenic acid A in Chrysanthemum and processed products.