上皮钙黏蛋白、雌激素受体α在子宫内膜癌变过程中的表达

Expression of E-cadherin and ERα during carcinogenesis of endometrium

目的探讨上皮钙黏蛋白(E-cadherin)、雌激素受体α(ERα)在子宫内膜癌变过程中的表达及临床意义。方法应用免疫组化En Vision二步法检测28例不典型增生子宫内膜(AH)和55例子宫内膜样腺癌(EA)组织中E-cadherin、ERα蛋白表达,另设18例增殖期子宫内膜组织作对照组。结果 1对照组、AH组、EA组组织中Ecadherin蛋白异常表达率分别为5.6%(1/18)、35.7%和60%,ERα阳性率分别为72.2%、53.6%和45.5%,ERα表达强度逐渐减弱,组间比较差异显著(均P<0.05)。2EA组中,E-cadherin蛋白表达与肿瘤侵犯肌层相关(P<0.05),与患者月经状态、组织学分级、临床分期无关(P>0.05);ERα蛋白表达与肿瘤组织学分级相关(P<0.01),与患者月经状态、肌层浸润及临床分期无关(P>0.05)。E-cadherin与ERα的表达呈正相关(P<0.05)。结论 Ecadherin和ERα改变可能参与了子宫内膜癌变过程,在EA的发生、发展过程中二者可能具有某种协同作用。

Purpose To investigate the expression and clinical significance of E-cadherin and ERα protein during carcinogenesis of endometrium. Methods The expression of E-cadherin and ERα proteins in endometrial atypical hyperplasia tissues（ n = 28）（ AH group）, endometrioid adenocarcinoma tissues（ n = 55）（ EA group） were examined by immunohistochemistry（ En Vision method）. Another 18 cases of proliferative endometrium tissues were used as a control group.Results In group of control,AH group and EA group,the abnormal expression rate of E-cadherin was 5. 6%,35. 7% and60. 0% respectively; The positive expression rates of ERα was 72. 2%,53. 6% and 45. 5% respectively and the signal intensity was decreasing. The differences among the three groups was atatistically significant（ P〈0. 05）. In EA group,the exprssion of E-cadherin was related to the muscle layer infiltration（ P〈0. 05）,but it was not associated with the menstrual status,histological grade and FIGO stage（ P〉0. 05）. The positive expression of ERα was related to the histological grade（ P〈0. 01）,but was not associated with menstrual status,muscle layer infiltration and FIGO stage（ P〉0. 05）. The expression of E-cadherin protein was positively related to that of ER（ P〈0. 05）. Conclusion The chang of E-cadherin and ERα protein may be involved in the pathogenesis of carcinogenesis of endometrium and may be associated with the pathogenesis and progression of endometrioid adenocarcinoma. Therefore,they may be has some synergistic effect in this process.