亚麻籽饼粕中亚麻蛋白的初步泡沫分离

PRELIMINARY SEPARATION OF FLAXSEED PROTEIN FROM FLAXSEED MEAL

以未脱胶亚麻籽饼粕为原料,采用泡沫分离法对亚麻蛋白分离工艺进行研究.以亚麻蛋白质量浓度、原料液p H值、装液量以及Na Cl浓度为因素进行响应面试验,得出泡沫分离的最佳工艺条件为:料液质量浓度是1.8 mg·m L-1,p H值为3.0,装液量是190 m L,Na Cl浓度为0.75%.在此条件下,亚麻蛋白的富集比为9.80,回收率为95.8%.研究结果表明:采用泡沫分离技术可以实现未脱胶亚麻籽饼粕中亚麻蛋白的分离纯化,是亚麻蛋白初级分离的一种有效方法.

Taking raw flaxseed meal as starting material,we studied the flaxseed protein separation process by foam separation method. We applied response surface methodology to study the optimum foam separation condition;and the optimum foam separation parameters were as follows： mass concentration of flaxseed meal solution 1.8 mg·mL^-1,pH 3.0,solution loading amount 190 mL,and NaCl mass fraction 0.75%. Under the optimum conditions, the enrichment ratio and the yield of flaxseed protein reached 9.80 and 95.8%,respectively. The result indicates the foam separation technology can realize separation and purification of flaxseed protein from raw flaxseed meal,and is effective in preliminary separation of flaxseed protein.