髓系抑制性细胞与诱导型一氧化氮合成酶在哮喘小鼠肺组织中的表达及意义

Expressions and significance of myeloid- derived suppressor cells and induced nitric oxide synthase in lung tissue of mice with asthma

目的:探讨髓系抑制性细胞(MDSCs)及诱导型一氧化氮合酶(iNOS)在哮喘小鼠肺组织中的表达及意义。方法:将BALB/C小鼠随机分成哮喘组及对照组,每组10只。用卵清蛋白(OVA)致敏、激发建立小鼠哮喘模型,用HE染色观察两组小鼠肺组织病理改变;用免疫组织化学(免疫组化)方法检测小鼠肺组织中MDSCs积分光密度值平均值;用RT-PCR检测小鼠肺组织中iNOS mRNA的表达。结果:哮喘组可见气道壁增厚,炎性细胞浸润,对照组则无明显变化。哮喘组小鼠肺组织MDSCs、iNOS mRNA的表达水平显著高于对照组,差异有统计学意义(P<0.05)。结论:哮喘小鼠肺组织中MDSCs的聚集增加,iNOS的表达增多,MDSCs通过表达iNOS来实现其在哮喘小鼠气道炎症、气道高反应及免疫抑制等方面的重要作用。

Objective： To explore the expressions and significance of myeloid - derived suppressor cells （MDSCs） and induced nitric oxide synthase （iNOS） in lung tissue of mice with asthma. Methods： Twenty BALB/C mice were randomly divided into asthma group and control group, ten mice in each group. The mice were sensitized and challenged with ovalbumin （OVA） to establish a mouse model of asthma, HE staining was used to observe pathological changes of lung tissues in mice in the two groups; immunohistochemical method was used to detect the average MDSCs integral optical density value; RT - PCR was used to detect iNOS mRNA expression in lung tissues of mice. Results： In asthma group, airway wall thickening and inflammatory cell infiltration were found; in control group, no obvious change was found. The expression levels of MDSCs and iNOS mRNA in lung tissues of mice in asthma group were statistically significantly higher than those in control group （ P 〈 0. 05 ） . Conclusion ： MDSCs aggregation and iNOS expression increased in lung tissue of mice with asthma; MDSCs plays important roles in airway inflammation, airway hyperresponsiveness and immunosuppression of mice with asthma by expression of iNOS.