摘要
以耐甲醇铜绿假单胞菌（Pseudomonas aeruginosa）S8为出发菌株,通过摇瓶发酵产脂肪酶,对发酵脂肪酶粗酶液进行酶学性质及固定化研究。结果表明：该脂肪酶反应的最适温度为35℃,最适p H值为7.0,在p H 6.0～8.0酶活较稳定。以硅藻土为载体,采用吸附法,对脂肪酶进行固定,脂肪酶的最佳固定化条件为：载体硅藻土与脂肪酶质量比为10,固定化温度为30℃,缓冲液p H值为7.5,固定化时间为2.5 h。
The lipase produced by Pseudomonas aeruginosa S8 through flask fermentation was studied in enzymatic properties and immobilization. The results showed that the optimal reaction temperature and opti- mum pH value were 35℃ and 7.0 respectively. The enzyme activity was stable at pH 6.0 - 8.0. The lipase was immoblilized on the carrier diatomite with absorption activity. The optimal immobilizing conditions were the mass ratio of carrier to enzymae as 10, immobilizing for 2.5 hours at 30℃ and pH value as 7.5.
出处
《山东农业科学》
2015年第2期54-57,共4页
Shandong Agricultural Sciences
基金
中国农业大学烟台研究院科研基金(项目编号:YT201102)
关键词
铜绿假单胞菌
脂肪酶
酶学性质
固定化
Pseudomonas aeruginosa
Lipase
Enzymatic properties
Immobilization
