玉米QR-001/QS-001组合抗粗缩病QTL初步分析

Preliminary QTL Analysis on Resistance of Maize Combination QR-001/QS-001 to Rough Dwarf Disease

本研究以QR-001/QS-001组合衍生的281个F2∶3家系为定位群体,在青岛和枣庄两个环境下进行非接种条件下玉米粗缩病抗性鉴定。应用完备区间作图法（ICIM）进行QTL分析,结果表明：在青岛环境下共检测到8个QTLs,分布在第1、2、3、4、6（2个）和8（2个）染色体上,单个QTL可以解释的表型变异在0.08%～32.25%之间;在枣庄环境下共检测到13个QTLs,分布在第1（3个）、2、3、4、5、6（2个）、7（2个）和8（2个）染色体上,单个QTL可以解释的表型变异在0.06%～35.61%之间。两环境下检测到2个通用主效QTLs,分别位于第1染色体umc2236-umc1278标记区间和第6染色体phi299852-umc1490标记区间,其在青岛环境下解释的表型变异分别为27.11%和32.25%,在枣庄环境下解释的表型变异分别为35.61%和27.77%。这两个区间可作为抗粗缩病候选基因的重要遗传位点开展精细定位。

The 281 F2：3 families derived from the cross of QR -001 with Qs -001 were used as target population. Their resistance to maize rough dwarf disease was identified under no vaccination condition in Qingdao and Zaozhuang. The QTL was analyzed by the method of inclusive composite interval mapping （ICIM）. The results showed that 8 QTLs, mapped on chromosome 1, 2, 3, 4, 6 （2 QTLs） and 8 （2 QTLs） , were detected out, and the phenotypie variation, which could be explained by every QTL, was from 0.08% to 32.25% in Qingdao. In Zaozhuang,13 QTLs mapped on chromosome 1 （3 QTLs）, 2, 3, 4, 5, 6 （2 QTLs）, 7 （2 QTLs）and 8 （2 QTLs） were detected out, and the phenotypic variation, which could be ex- plained by every QTL, was from 0.06% to 35.61%. Two common QTLs with main effect were detected out in the two environments, which were mapped at umc2236 -umc1278 and phi299852 -umc1490 SSR marker in- tervals. The phenotypie variations explained in Qingdao and Zaozhuang were 27.11% , 32.25% and 35.61% , 27.77% respectively. These two marker intervals could be used as important genetic loci of candadite genes resisting maize rough dwarf disease to carry out the fine mapping.