基于叶绿体psbA-trnH序列和trnL-F序列的牡丹野生种间关系

Phylogenetic Relationships of Paeonia Sect. Moutan Based on Chloroplast psb A-trn H and trn L- F Sequences

对牡丹组全部9个野生种15个居群及凤丹的叶绿体psb A-trn H和trn L-F序列进行测序,并采用邻位相连法分别构建了psb A-trn H序列和trn L-F序列的系统发育树。结果显示:1)两段序列的基因树均支持牡丹组划分为两个亚组的分类方法,与前人的研究结果一致。2)psb A-trn H序列基因树表明大花黄牡丹与黄牡丹的亲缘关系最近,这与大花黄牡丹曾被确定为黄牡丹的一个变种的历史一致。3)革质花盘亚组内,基于psb A-trn H和trn L-F序列的研究结果大体一致,分歧主要在四川牡丹的地位问题。4)psb A-trn H序列和trn L-F序列基因树分别表明杨山牡丹与凤丹具有最近或较近的亲缘关系。

The psb A-trn H sequences and trn L-F sequences of nine species in Sect. Moutan DC. and one cultivar Fengdan＇were sequenced,then the phylogenetic trees based on two sequences were constructed by Neighbor-joining（ NJ） methods in MEGA 6. 06,respectively. The results showed as follows： 1） Nine species in Sect. Moutan DC. were divided into two Subsects from two phylogenetic trees,consistent with previous research results. 2） Paeonia ludlowii,that had been identified as a variation of Paeonia lutea,was most closely related to Paeonia lutea based on psb A-trn H sequences. 3） The results were largely consistent in Subsect. Vaginatae,except the relationship between Paeonia decomposita and other four species bases on two sequences. 4） Paeonia ostii had the closest or very closer relationship with the cultivar Fengdan＇based on psb A-trn H sequences and trn L-F sequences,respectively.