摘要
以铜绿微囊藻为试验对象,研究其在不同温度、光照、氮磷浓度条件下生长和产毒的情况.通过计算铜绿微囊藻细胞数来反应其生物量的变化趋势,用高效液相色谱仪测定藻毒素-LR的浓度来反应藻细胞产毒变化.结果表明,铜绿微囊藻在30℃、1 000 Lux时生长最快,而在25℃、500 Lux时产毒最多;铜绿微囊藻生物量和产毒量随着总氮浓度的升高而增多;磷是一种限制性营养因子,较低浓度时就可以满足铜绿微囊藻的生长和产毒;最适合铜绿微囊藻产毒的氮磷比为100∶1.
The environmental factor such as temperature, light, nitrogen and phosphorus were studies to deter mine their effects on the growth and microcystin production of Microcystis aeraginosa. Growth was deter mined by calculating algae cell, while microcystin-LR was analyzed by high-performance liquid chromatography. The result indicated that the most favorable conditions for the growth was the temperature of 30 ℃ and the light intensity of 1 000 Lux, but it produced the most toxin under 25 ℃、500 Lux. In addition, M. aeraginosa amount and microcystin-LR content increased with the increase of nitrogen. Phosphorus is a probable limitation nutrient factor, and a low concentration will satisfy the growth and toxin production of M. aeraginosa. The N：P atomic ratio at 100：1 was deter mined as the optimum for growth and toxin production.
出处
《辽宁大学学报（自然科学版）》
CAS
2015年第1期85-90,共6页
Journal of Liaoning University：Natural Sciences Edition
关键词
微囊藻毒素-LR
温度
光照强度
氮
磷
microcystins-LR
temperature
rumination
nitrate
phosphorus
