温州地区672名献血者Kidd血型抗原分布调查

Investigation on the distribution of Wenzhou area blood donors,group Kidd blood antigens

目的:调查温州地区献血者Kidd血型人群分布状况,对Kidd血型不配合的输血风险进行评估。方法:随机抽取2013-03-2013-09温州地区献血者的血液标本672名作为研究对象,采用微量板法进行Kidd血型抗原的鉴定,再用血清学方法对可疑的Kidd血型抗原进行确认。结果:672名献血者中,Jka和Jkb的基因频率分别为0.442 8和0.557 2。Jka和Jkb不配合的机率分别为0.214 1和0.157 6。经χ2检验,Kidd血型的观察值与期望值之间比较差异无统计学意义(P>0.05),Hardy-Weinberg吻合度良好。结论:温州地区献血者Kidd血型抗原分布具有多态性,在临床随机输血中Jka和Jkb不配合的机率合计为0.371 7,应引起高度重视,以保障临床输血安全。

Objective：To investigate the distribution of Wenzhou area blood donors,Kidd blood group antigens and assess transfusion mismatch risk of Kidd blood group.Method：From March 2013 to September 2013,randomly selecting 672 blood samples from Wenzhou area blood donors identified Kidd blood group antigens by microplate method.Then these suspicious Kidd blood group antigens were confirmed by serological method.Result：In 672 blood donors,the phenotype distribution of Kidd blood group was as following：Jk（a＋b＋）（47.77%）〉Jk（a-b＋）（31.84%）〉Jk（a＋b-）（20.39%）,no Jk（a-b-）was found,The gene frequencyes of Jka and Jkb was0.442 8and 0.557 2respectively.The rate of Jka and Jkb mismatches was 0.2141 and 0.1576 respectively.The differerence between the expected and observed values of the Kidd blood group was no statistical significance（P〉0.05）.Hardy-Weinberg,the goodness of fit test results were good.Conclusion：There is great different distribution among the gene frequencies of Kidd blood group antigens.The rate of Jka and Jkb mismatches in randomized clinical blood transfusion total 0.371 7,Attention should be paid to protect the safety of clinical transfusion.