不同品种橡胶树花药愈伤组织诱导、分化及植株再生的比较

Comparison of Callus Induction, Somatic Embryogenesis and Plant Regeneration from Different Clones of Hevea brasiliensis Anther

为优化橡胶树花药体细胞胚诱导及植株再生体系,为橡胶树分子育种提供技术支撑,以橡胶树品种‘云研73-477’、‘云研73-46’、‘热研8-79’、‘云研77-4’、‘RRII105’、‘热垦525’的花药为材料,比较不同品种在相同培养条件下愈伤组织诱导、体胚发生能力及植株再生频率。结果表明,6个品种的花药均能脱分化形成愈伤组织,但不同品种间存在显著差异,诱导率最高的是‘RRII105’和‘热垦525’,分别为93.33%、90.33%,最低的是‘云研77-4’,为31.33%;体胚发生能力在不同品种间也有较大差异,‘云研73-477’体胚诱导率最高达76%,‘云研73-46’没有分化出体细胞胚;植株再生频率较高的品种有‘热垦525’和‘云研73-477’,分别为75.38%、54.35%,‘云研73-46’和‘云研77-4’无再生植株。

In order to optimize the somatic embryogenesis and plant regeneration system of anther and providea molecular breeding protocol for Hevea brasiliensis, callus induction, somatic embryogenesis and plantregeneration were compared under the same condition using anthers from six clones‘Yunyan 73-477＇,‘Yunyan 73-46＇,‘Reyan 8-79＇,‘Yunyan 77-4＇,‘RRII105＇and‘Reken 525＇of H. brasiliensis. Theresults indicated that the anthers from six clones were all able to dedifferentiate into callus tissues. But therewere significant differences among different clones,‘RRII105＇and‘Reken 525＇showed the highest callusinduction rates of 93.33% and 90.33%, respectively. The lowest differentiation rate was 31.33%, which wasfound in‘Yunyan 77-4＇. The somatic embryogenesis of different clones was significantly different, the highestdifferentiation rate was 76% which was found in‘Yunyan 73-477＇, and the clones of‘Yunyan 73-46＇had nosomatic embryo.‘Reken 525＇and‘Yunyan 73-477＇showed high plant regeneration rates of 75.38% and54.35%, respectively, the clones of‘Yunyan 73-46＇and‘Yunyan 77-4＇had no regeneration plant.