基于再生不定芽的蝴蝶兰继代扩繁体系建立

Building of the Phalaenopsis' Subculture Multiplication System via Regenerating Adventitious Buds

为降低蝴蝶兰通过再生原球茎途径频繁继代培养可能出现的变异及玻璃化风险、减轻继代培养中的褐化,以蝴蝶兰花梗茎段再生的营养芽为试验材料,进行基于再生不定芽的继代扩繁及抗褐化研究。结果显示,以5节花梗的中间腋芽茎段为材料,在VW+6-BA 5 mg/L+NAA 0.1 mg/L+蔗糖30 g/L培养基上进行启动培养,能获得最高的营养芽诱导率。继代培养中,TDZ促进再生不定芽的效果比6-BA强。茎芽经过"切叶"处理,在培养基VW+TDZ 1 mg/L+300 mg/L柠檬酸+10 g/L蔗糖+15%椰汁,或培养基VW+6-BA 5 mg/L+NAA 0.1 mg/L+300 mg/L柠檬酸+10 g/L蔗糖+15%椰汁上进行继代培养,能获得最佳的继代扩繁效果。笔者研究认为初步建立了基于再生不定芽的蝴蝶兰继代扩繁体系。

To reduce the possibilities of genetic variation or vitrification during continuous subculture viaregenerating protocorms and to alleviate the browning, a Phalaenopsis＇ subculture technique via regeneratingadventitious buds from vegetative buds produced from the flower stalks and applying anti-browning agent wasstudied. The results showed that： the highest vegetative bud induction rate was achieved when the middleauxiliary bud stem segments from the five-nodes flower stalks were inoculated in the medium of VW＋ 6-BA5 mg/L ＋ NAA 0.1 mg/L ＋ sugar 30 g/L. In subculture, TDZ was more effective than 6-BA in stimulatingadventitious buds regeneration. The best subculture multiplication efficiency was achieved when theinoculating buds treated with‘cutting leaf＇were cultured in the medium VW ＋ TDZ 1 mg/L ＋ citric acid300 mg/L＋ sugar 10 g/L＋ coconut water 15%, or in the medium VW＋ 6-BA 5 mg/L＋ NAA 0.1 mg/L＋ citricacid 300 mg/L ＋ sugar 10 g/L ＋ coconut water 15%. Through studies, the Phalaenopsis＇ subculturemultiplication system was preliminarily set up via regenerating adventitious buds.