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陈年普洱茶香气成分及清除DPPH自由基活性研究 被引量:9

Analysis of Aroma Components and DPPH· Scavenging Activity of Aged Pu-erh Tea
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摘要 为研究陈年普洱茶的特征风味成分及生物活性,采用顶空-固相微萃取-气质联用法测定了3种陈年普洱茶中挥发性物质,结合质谱和保留指数共鉴定出57种化合物,其中甲氧基酚类、醇类及酮类化合物为主要香气成分。同时采用比色法测得3种普洱茶的沸水提取物的茶多酚含量分别为(41.91±1.98)、(43.84±2.56)及(41.28±2.57)mg GAE/(g·DW),并对3种普洱茶的1,1-二苯基苦基苯肼(DPPH·)自由基清除效果进行研究。结果表明3种普洱茶均具有较好的清除DPPH自由基能力,且呈剂量依赖性,测得3种普洱茶提取物清除DPPH·自由基的IC50分别为(129.03±0.25)、(139.47±1.58)及(152.57±3.34)mg GAE/L。 The aim of this study was to investigate the flavor characteristics and bioactivity of aged Pu-erh tea.Volatile compounds from aged Pu-erh tea were measured using solid-phase micro-extraction(SPME) and gaschromatography-mass spectrometry(GC-MS). Results showed that a total of 57 volatile compounds wereidentified by matching EI mass spectra and retention indices with the literature data. Alcohols,methoxy-phenolic and ketones were the three major aromatic groups. The total phenolic contents of three typesof Pu-erh tea were(41.91±1.98),(43.84±2.56) and(41.28±2.57) milligrams of gallic acid equivalent per gramof dried weight [mg GAE/(g·DW)]. The effects of Pu-erh tea extracts on the 2,2-diphenyl-1-picrylhydrazylradical(DPPH ·) scavenging activity were evaluated. The results showed that all types Pu-erh tea extractsexhibited the relative strong DPPH· scavenging activity and in dosage-dependent modes. The concentration ofsample required for 50% inhibition of DPPH ·(IC50) were(129.03 ± 0.25),(139.47 ± 1.58) and(152.57±3.34) mg GAE/L, respectively.
出处 《中国农学通报》 2015年第4期274-279,共6页 Chinese Agricultural Science Bulletin
基金 国家"948"项目"高效新型微生物资源引进与创新"(2011-G25)
关键词 普洱茶 香气成分 固相微萃取 气质联用 1 1-二苯基苦基苯肼 Pu-erh tea aroma compounds SPME GC-MS DPPH
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