探讨miRNA表达比在多发性骨髓瘤中的预测作用

Studies on the roles of miRNA expression forecast in multiple myeloma

目的探讨miRNA(微小RNA)表达比在多发性骨髓瘤患者血浆中的预测作用。方法收集24例MM患者及30例正常对照的血浆标本,运用高通量芯片的方法将差异表达的miRNA进行筛选;对分析结果进行qRT-PCR验证;用ROC曲线分析了miRNA表达比,找出最具预测潜能的组合型标志物。结果与正常血浆标本相比,基因芯片检测出178个差异表达的miRNA,选取其中4个miRNAs在24例MM血浆标本和30例正常血浆标本进行验证。其中miR-21在多发性骨髓瘤中比在正常人中有明显的高表达,而miR-199a-5p,miR-16和miR-125a-5p在多发性骨髓瘤中则是低表达;miR-21/miR-199a-5p的表达比表现出很高的灵敏度和特异度,可以作为诊断多发性骨髓瘤的组合型生物标志物。结论 miR-21/miR-199a-5p表达比或许可以成为预测多发性骨髓瘤的生物标志物。

Objective We sought to identify microRNA altered in multiple myelomaand determine that microRNA ratio is an predictive tool for multiple myeloma.Methods The plasma of MM patients and 30 normal controls were choosed and cofirmed,Respectively.The miRNA expression profiles were examined with Illumina Bead Chips.The results were verified by qRT-PCR.The miRNA expression ratio was analysed by ROC curve,aiming to find the most predictive potential of combination-type markers.Results 178 miRNAs were found to be significantly deregulated in the plasma of 24 MM patients and 30 normal controls plasma samples.miR-21 were significantly higher than in normal plasma sample,while miR-199a-5p,miR-16 and miR-125a-5p showed low expression;Furthermore,the ratio of miR-21/miR-199a-5p showed a high sensitivity and specificity for disease predicition.Conclusion These results indicate that miR-21/miR-199a-5p ratio could be used as diagnostic biomarker in multiple myeloma.